Influence of different substrates on the formation and characteristics of aerobic granules in sequencing batch reactors

The effects of diffcrcnt Substrates on the aerobic granulation process were studied using laboratory-scale sequencing batch reactors (SBRs). Four parallel granules sequencing batch reactors (GSBR): R₁, R₂, R₃, and R₄ were fed with acetate, glucose, peptone and fecula, respectively. Stable aerobic granules were successfblly cultivated in R₁, R₂, R₄, and smaller granules less than 500 μm were formed in R₃. Morphology and the physic-chemical characteristics of acrobic granules fed with diffcrent carbon substrates were investigated by the four reactors operated under the same pressure. The aerobic granules in the four reactors were observed and found that peptone was the most stable one due to its good settleability even after a sludge age as short as 10 d. A strong correlation was testified between the characteristics of aerobic granules and the properties of carbon substrates. The stability of aerobic granules was affected by extracellular polymer substances (EPS) derived from microorganism growth during fcast time fcd with different carbon substrates, and the influence of the property of storage substance was greater than that of its quantity. Optimal carbon substrates, which are helpful in the cultivation and retention of well-settling granules and in the enhancement of thc overall ability of thc aerobic granules reactors, were found.