Incorporation of tellurocysteine into glutathione transferase generates high glutathione peroxidase efficiency

Xiaoman Liu; Louis A. Silks; Cuiping Liu; Morgane Ollivault-Shiflett; Xin Huang; Jing Li; Guimin Luo; Ya Ming Hou; Junqiu Liu; Jiacong Shen

doi:10.1002/anie.200805365

Incorporation of tellurocysteine into glutathione transferase generates high glutathione peroxidase efficiency

Xiaoman Liu^*
, Louis A. Silks
, Cuiping Liu
, Morgane Ollivault-Shiflett
, Xin Huang
, Jing Li
, Guimin Luo
, Ya Ming Hou
, Junqiu Liu
, Jiacong Shen

^*Corresponding author for this work

Jilin University
Los Alamos National Laboratory
Thomas Jefferson University

Research output: Contribution to journal › Article › peer-review

79 Scopus citations

Abstract

(Chemical Equation Presented) A rival to native peroxidase! An existing binding site for glutathione was combined with the catalytic residue tellurocysteine by using an auxotrophic expression system to create an engineered enzyme that functions as a glutathione peroxidase from the scaffold of a glutathione transferase (see picture). The catalytic activity of the telluroenzyme in the reduction of hydroperoxides by glutathione is comparable to that of native glutathione peroxidase.

Original language	English
Pages (from-to)	2020-2023
Number of pages	4
Journal	Angewandte Chemie - International Edition
Volume	48
Issue number	11
DOIs	https://doi.org/10.1002/anie.200805365
State	Published - 2 Mar 2009
Externally published	Yes

Keywords

Enzyme models
Glutathione peroxidase
Protein design
Tellurium
Tellurocysteine

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10.1002/anie.200805365

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title = "Incorporation of tellurocysteine into glutathione transferase generates high glutathione peroxidase efficiency",

abstract = "(Chemical Equation Presented) A rival to native peroxidase! An existing binding site for glutathione was combined with the catalytic residue tellurocysteine by using an auxotrophic expression system to create an engineered enzyme that functions as a glutathione peroxidase from the scaffold of a glutathione transferase (see picture). The catalytic activity of the telluroenzyme in the reduction of hydroperoxides by glutathione is comparable to that of native glutathione peroxidase.",

keywords = "Enzyme models, Glutathione peroxidase, Protein design, Tellurium, Tellurocysteine",

author = "Xiaoman Liu and Silks, \{Louis A.\} and Cuiping Liu and Morgane Ollivault-Shiflett and Xin Huang and Jing Li and Guimin Luo and Hou, \{Ya Ming\} and Junqiu Liu and Jiacong Shen",

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doi = "10.1002/anie.200805365",

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T1 - Incorporation of tellurocysteine into glutathione transferase generates high glutathione peroxidase efficiency

AU - Liu, Xiaoman

AU - Silks, Louis A.

AU - Liu, Cuiping

AU - Ollivault-Shiflett, Morgane

AU - Huang, Xin

AU - Li, Jing

AU - Luo, Guimin

AU - Hou, Ya Ming

AU - Liu, Junqiu

AU - Shen, Jiacong

PY - 2009/3/2

Y1 - 2009/3/2

N2 - (Chemical Equation Presented) A rival to native peroxidase! An existing binding site for glutathione was combined with the catalytic residue tellurocysteine by using an auxotrophic expression system to create an engineered enzyme that functions as a glutathione peroxidase from the scaffold of a glutathione transferase (see picture). The catalytic activity of the telluroenzyme in the reduction of hydroperoxides by glutathione is comparable to that of native glutathione peroxidase.

AB - (Chemical Equation Presented) A rival to native peroxidase! An existing binding site for glutathione was combined with the catalytic residue tellurocysteine by using an auxotrophic expression system to create an engineered enzyme that functions as a glutathione peroxidase from the scaffold of a glutathione transferase (see picture). The catalytic activity of the telluroenzyme in the reduction of hydroperoxides by glutathione is comparable to that of native glutathione peroxidase.

KW - Enzyme models

KW - Glutathione peroxidase

KW - Protein design

KW - Tellurium

KW - Tellurocysteine

UR - https://www.scopus.com/pages/publications/61849128784

U2 - 10.1002/anie.200805365

DO - 10.1002/anie.200805365

M3 - 文章

C2 - 19199319

AN - SCOPUS:61849128784

SN - 1433-7851

VL - 48

SP - 2020

EP - 2023

JO - Angewandte Chemie - International Edition

JF - Angewandte Chemie - International Edition

IS - 11

ER -

Incorporation of tellurocysteine into glutathione transferase generates high glutathione peroxidase efficiency

Abstract

Keywords

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