In vivo Raman imaging of mice ear

Raman spectra had been used to the image analysis of spatial distribution of chemical composition by scanning the laser spot across interest area of mice ear at different depth. By selecting the bands at 1125 cm^-1 (blood sugar), 1300 cm^-1 (lipids), 1549 cm^-1 (hemoglobin) and 1660 cm^-1 (protein) to calculate their peak areas, and using these data to generate 2D, and 3D images, we have demonstrated that the Raman imaging can yield 3D spatially biochemical information in a living tissue. This technique provides a new way for the study of biochemical molecule's distribution in vivo.