In vitro assessment of immunomodulating activity of the two Lactobacillus strains isolated from traditional fermented milk

Yanfeng Tuo; Lanwei Zhang; Xue Han; Ming Du; Yingchun Zhang; Huaxi Yi; Weiqin Zhang; Yuehua Jiao

doi:10.1007/s11274-010-0482-8

In vitro assessment of immunomodulating activity of the two Lactobacillus strains isolated from traditional fermented milk

Yanfeng Tuo
, Lanwei Zhang^*
, Xue Han
, Ming Du
, Yingchun Zhang
, Huaxi Yi
, Weiqin Zhang
, Yuehua Jiao

^*Corresponding author for this work

Gansu Agricultural University
Harbin Institute of Technology
Tarim University

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

The objective of this study was to evaluate the in vitro immunomodulating capacity of Lactobacillus coryniformis subsp torquens T3L (L. coryniformis T3L) isolated from traditional fermented yak's milk in Tibet, China, and Lactobacillus paracasei supsp. paracasei M5L (L. paracasei M5L)isolated from kumiss in Sinkiang, China was used as control. The effects of live bacteria, cell wall and genomic DNA of the two Lactobacillus strains on human peripheral blood mononuclear cells (PBMCs) proliferation, production of interleukin-12 (IL-12 p70), interferon gamma (IFN-γ) and tumor necrosis factor alpha (TNF-α), and natural killer (NK) cell activity were assessed. The live bacteria, cell wall and genomic DNA of the two lactobacilli exerted proliferative effects on PBMCs. Live bacteria at 1 × 10⁶ c.f.u. ml^-1, cell wall at 20 μg protein ml^-1 and DNA at 50 μg DNA ml^-1 of the strainS induced the secretion of IL-12 (p70), IFN-γ and TNF-α by PBMCs. NK cell activities increased after cultivation of PBMCs with live bacteria, cell wall and DNA of the strains. Overall, these results demonstrate that the live bacteria, cell wall and genomic DNA of the two Lactobacillus strains exhibit immunomodulating activity.

Original language	English
Pages (from-to)	505-511
Number of pages	7
Journal	World Journal of Microbiology and Biotechnology
Volume	27
Issue number	3
DOIs	https://doi.org/10.1007/s11274-010-0482-8
State	Published - Mar 2011

Keywords

Immunomodulating activity
Lactobacillus
Natural killer cell activity
Peripheral blood mononuclear cells
Traditional fermented yak's milk

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10.1007/s11274-010-0482-8

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title = "In vitro assessment of immunomodulating activity of the two Lactobacillus strains isolated from traditional fermented milk",

abstract = "The objective of this study was to evaluate the in vitro immunomodulating capacity of Lactobacillus coryniformis subsp torquens T3L (L. coryniformis T3L) isolated from traditional fermented yak's milk in Tibet, China, and Lactobacillus paracasei supsp. paracasei M5L (L. paracasei M5L)isolated from kumiss in Sinkiang, China was used as control. The effects of live bacteria, cell wall and genomic DNA of the two Lactobacillus strains on human peripheral blood mononuclear cells (PBMCs) proliferation, production of interleukin-12 (IL-12 p70), interferon gamma (IFN-γ) and tumor necrosis factor alpha (TNF-α), and natural killer (NK) cell activity were assessed. The live bacteria, cell wall and genomic DNA of the two lactobacilli exerted proliferative effects on PBMCs. Live bacteria at 1 × 106 c.f.u. ml-1, cell wall at 20 μg protein ml-1 and DNA at 50 μg DNA ml-1 of the strainS induced the secretion of IL-12 (p70), IFN-γ and TNF-α by PBMCs. NK cell activities increased after cultivation of PBMCs with live bacteria, cell wall and DNA of the strains. Overall, these results demonstrate that the live bacteria, cell wall and genomic DNA of the two Lactobacillus strains exhibit immunomodulating activity.",

keywords = "Immunomodulating activity, Lactobacillus, Natural killer cell activity, Peripheral blood mononuclear cells, Traditional fermented yak's milk",

author = "Yanfeng Tuo and Lanwei Zhang and Xue Han and Ming Du and Yingchun Zhang and Huaxi Yi and Weiqin Zhang and Yuehua Jiao",

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AU - Tuo, Yanfeng

AU - Zhang, Lanwei

AU - Han, Xue

AU - Du, Ming

AU - Zhang, Yingchun

AU - Yi, Huaxi

AU - Zhang, Weiqin

AU - Jiao, Yuehua

PY - 2011/3

Y1 - 2011/3

N2 - The objective of this study was to evaluate the in vitro immunomodulating capacity of Lactobacillus coryniformis subsp torquens T3L (L. coryniformis T3L) isolated from traditional fermented yak's milk in Tibet, China, and Lactobacillus paracasei supsp. paracasei M5L (L. paracasei M5L)isolated from kumiss in Sinkiang, China was used as control. The effects of live bacteria, cell wall and genomic DNA of the two Lactobacillus strains on human peripheral blood mononuclear cells (PBMCs) proliferation, production of interleukin-12 (IL-12 p70), interferon gamma (IFN-γ) and tumor necrosis factor alpha (TNF-α), and natural killer (NK) cell activity were assessed. The live bacteria, cell wall and genomic DNA of the two lactobacilli exerted proliferative effects on PBMCs. Live bacteria at 1 × 106 c.f.u. ml-1, cell wall at 20 μg protein ml-1 and DNA at 50 μg DNA ml-1 of the strainS induced the secretion of IL-12 (p70), IFN-γ and TNF-α by PBMCs. NK cell activities increased after cultivation of PBMCs with live bacteria, cell wall and DNA of the strains. Overall, these results demonstrate that the live bacteria, cell wall and genomic DNA of the two Lactobacillus strains exhibit immunomodulating activity.

AB - The objective of this study was to evaluate the in vitro immunomodulating capacity of Lactobacillus coryniformis subsp torquens T3L (L. coryniformis T3L) isolated from traditional fermented yak's milk in Tibet, China, and Lactobacillus paracasei supsp. paracasei M5L (L. paracasei M5L)isolated from kumiss in Sinkiang, China was used as control. The effects of live bacteria, cell wall and genomic DNA of the two Lactobacillus strains on human peripheral blood mononuclear cells (PBMCs) proliferation, production of interleukin-12 (IL-12 p70), interferon gamma (IFN-γ) and tumor necrosis factor alpha (TNF-α), and natural killer (NK) cell activity were assessed. The live bacteria, cell wall and genomic DNA of the two lactobacilli exerted proliferative effects on PBMCs. Live bacteria at 1 × 106 c.f.u. ml-1, cell wall at 20 μg protein ml-1 and DNA at 50 μg DNA ml-1 of the strainS induced the secretion of IL-12 (p70), IFN-γ and TNF-α by PBMCs. NK cell activities increased after cultivation of PBMCs with live bacteria, cell wall and DNA of the strains. Overall, these results demonstrate that the live bacteria, cell wall and genomic DNA of the two Lactobacillus strains exhibit immunomodulating activity.

KW - Immunomodulating activity

KW - Lactobacillus

KW - Natural killer cell activity

KW - Peripheral blood mononuclear cells

KW - Traditional fermented yak's milk

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M3 - 文章

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SN - 0959-3993

VL - 27

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JO - World Journal of Microbiology and Biotechnology

JF - World Journal of Microbiology and Biotechnology

IS - 3

ER -

In vitro assessment of immunomodulating activity of the two Lactobacillus strains isolated from traditional fermented milk

Abstract

Keywords

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