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In situ comparative studies on subnucleolar distribution and configuration of plant rDNA

  • Hong Long
  • , Jie He
  • , Haijing Sun
  • , Shui Hao
  • , Mingda Jiao*
  • *Corresponding author for this work
  • Huazhong Agricultural University
  • Northeast Normal University

Research output: Contribution to journalArticlepeer-review

Abstract

The distribution and configurations of nucleolar DNA in Pisum sativum L., Allium sativum L., Triticum aestivum L. were analyzed by specific cytochemical staining using NAMA-Ur. It has been observed that in the nucleoli of different plant species, the DNA occupied different positions in different areas, which may imply a different status and strategy of rDNA transcription. Our results showed irregular clumps of rDNA surrounding FCs in semi-circular formations in P. sativum and T. aestivum, indicating a regular pattern of rDNA distribution and supporting the helix model of rDNA configuration. The rDNA was condensed in some regions and uncondensed in others. Nucleolus-associated chromatin extended from outside the nucleolus to the periphery of the FCs via nucleolar channels, which suggests a possible origin for nucleolar DNA.

Original languageEnglish
Pages (from-to)405-410
Number of pages6
JournalMicron
Volume39
Issue number4
DOIs
StatePublished - Jun 2008

Keywords

  • Allium sativum L.
  • In situ structure
  • Nucleolus
  • Nucleus
  • Pisum sativum L.
  • Triticum aestivum L
  • rDNA

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