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Identification of one polysaccharide gene and analysis of differentially expressed proteins from bioflocculant producing strain of F2

  • Bing Yu*
  • , Fang Ma
  • , Peishi Qi
  • , Ang Li
  • , Lili Wang
  • *Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

F2 is one of two compound bioflocculant producing strains with good genetic stability and high flocculating activity. The core sequence of exoL was amplified from the F2 genome by the method of polymerase chain reaction, identifying that the effective polysaccharide component of bioflocculant produced by F2 was succinoglycan. The changes of protein complexes of F2 grown in minimal media versus bioflocculant media were researched by SDS-PAGE, indicating that in bioflocculant media compared with minimal media, the relative content of two protein complexes were obviously increased and the relative content of one protein complexes was obviously decreased in gels. It was three differentially expressed protein complexes that can probably induce the production of large amount of the effective polysaccharide component of bioflocculant produced by F2. The research can provide a step to reveal the molecular flocculating mechanisms of compound bioflocculant producing strains.

Original languageEnglish
Title of host publicationEnvironment Materials and Environment Management
Pages305-310
Number of pages6
DOIs
StatePublished - 2010
Event2010 International Conference of Environment Materials and Environment Management, EMEM 2010 - Harbin, China
Duration: 24 Jul 201025 Jul 2010

Publication series

NameAdvanced Materials Research
Volume113-116
ISSN (Print)1022-6680

Conference

Conference2010 International Conference of Environment Materials and Environment Management, EMEM 2010
Country/TerritoryChina
CityHarbin
Period24/07/1025/07/10

Keywords

  • Bioflocculant producing strain of F2
  • Compound bioflocculant
  • ExoL gene
  • Protein complexes
  • SDS-PAGE
  • Succinoglycan

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