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Identification of a highly efficient aerobic denitrifying bacterium in SBR and denitrification optimization

  • Cong Du
  • , Chong Wei Cui
  • , Sheng Nan Shi
  • , Fang Ma*
  • *Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Aerobic denitrification process is attracted more attention to enhance nitrogen removal technology. Aerobic denitrifying bacteria present excelled abilities with fast growth rate and denitrifying speed, though they are not dominant in most practical processes. A high-efficiency strain was enriched in SBR from aerobic activated sludge by the way of intermittent aeration and continuous aeration combination. The strain was determined to Pseudomonas stutzeri T13 with ability of TN removal 90% and nitrate removal 97%. The limiting factors for aerobic denitrifying efficiency were optimized to temperature=31 °C,pH=7.11 and DO=2.5 mg/L using response surface methodology. A total of 32 genes are related to nitrogen removal in strain T13. And 10 related to nitrate reductase, especially including 3 genes encoding the periplasmic nitrate reductase, playing important role to aerobic denitrification. It gave good understanding to supply effective technological supports for aerobic denitrification process.

Original languageEnglish
Title of host publicationAdvances in Environmental Technologies III
PublisherTrans Tech Publications Ltd
Pages376-382
Number of pages7
ISBN (Print)9783038351351
DOIs
StatePublished - 2014
Event3rd International Conference on Energy and Environmental Protection, ICEEP 2014 - Xi'an, China
Duration: 26 Apr 201428 Apr 2014

Publication series

NameAdvanced Materials Research
Volume955-959
ISSN (Print)1022-6680
ISSN (Electronic)1662-8985

Conference

Conference3rd International Conference on Energy and Environmental Protection, ICEEP 2014
Country/TerritoryChina
CityXi'an
Period26/04/1428/04/14

Keywords

  • Aerobic denitrification
  • Nitrogen removal
  • Optimal conditions
  • Response surface methodology
  • SBR

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