Highly effective enhancement of waste activated sludge dewaterability by altering proteins properties using methanol solution coupled with inorganic coagulants

Proteins are the dominant organic component of extracellular polymeric substances (EPS) in waste activated sludge (WAS), and play an important role during sludge dewatering processes. Methanol is a polar hydrophilic reagent and can denature proteins, which suggested to us that the modification of protein configurations with methanol could improve sludge dewatering performance. In this study, methanol was used to precondition WAS prior to adding inorganic coagulants for dewatering enhancement. The morphology and EPS properties (especially of proteins) were investigated to analyze and explain the effects of methanol in the sludge conditioning process. The results show that methanol performed much better than traditional inorganic coagulants in improving sludge dewaterability in term of specific resistance to filtration (SRF) and cake solid content (CSC). Extractable proteins in EPS increased to a maximum when the concentration of methanol reached 40% (w/w) because cell membranes were destroyed and intracellular substances and water were released. Floc protein content was reduced with the further increase in methanol concentration due to protein precipitation. Confocal laser scanning microscopy analysis indicated that proteins precipitated and formed larger aggregates because methanol destroyed both the hydration shell and the hydrophobic clusters of proteins and expanded the protein tertiary structure to release interstitial water and bound water. The combination treatment of methanol and inorganic coagulants (PAC or FeCl₃) showed significant synergetic effects on enhancing sludge dewatering and cake drying. In practical applications, methanol from the dewatering sludge can be returned to the biochemical pool and used as the carbon source for nitrogen removal in the denitrification process. This integrated process is appropriate for sludge final disposal technologies that have high energy demands, such as incineration and pyrolysis. This paper describes a novel approach to improving sludge dewaterability through the alteration of protein properties by use of physiochemical techniques.