Heterologous expression of transaldolase gene tal from saccharomyces cerevisiae in fusarium oxysporum for enhanced bioethanol production

Jin Xia Fan; Xiao Xue Yang; Jin Zhu Song; Xiao Mei Huang; Zhong Xiang Cheng; Lin Yao; Olivia S. Juba; Qing Liang; Qian Yang; Margaret Odeph; Yan Sun; Yun Wang

doi:10.1007/s12010-011-9191-5

Heterologous expression of transaldolase gene tal from saccharomyces cerevisiae in fusarium oxysporum for enhanced bioethanol production

Jin Xia Fan
, Xiao Xue Yang
, Jin Zhu Song^*
, Xiao Mei Huang
, Zhong Xiang Cheng
, Lin Yao
, Olivia S. Juba
, Qing Liang
, Qian Yang
, Margaret Odeph
, Yan Sun
, Yun Wang

^*Corresponding author for this work

Faculty of Life Sciences and Medicine

Harbin Institute of Technology

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

The filamentous fungus Fusarium oxysporum is known for its ability to ferment xylose-producing ethanol. However, efficiency of xylose utilization and ethanol yield was low. In this study, the transaldolase gene from Saccharomyces cerevisiae has been successfully expressed in F. oxysporum by an Agrobacterium tumefaciens-mediated transformation method. The enzymatic activity of the recombinant fungus (cs28pCAM-Sctal4) was 0.195 times higher than that of the wild-type strain (cs28). The recombinant strain also exhibited a 28.83% increase in ethanol yield on xylose media compared to the parental strain. Enhanced ethanol production and a reduction in the biomass were observed during xylose fermentation. Ethanol yield from rice straw by simultaneous saccharification and fermentation with cs28pCAM-Sctal4 was 0.25 gg^-1 of rice straw. The transgenic strain of F. oxysporum cs28pCAM-Sctal4 might therefore have potential applications in industrial bioenergy production.

Original language	English
Pages (from-to)	1023-1036
Number of pages	14
Journal	Applied Biochemistry and Biotechnology
Volume	164
Issue number	7
DOIs	https://doi.org/10.1007/s12010-011-9191-5
State	Published - Aug 2011

Keywords

Bioethanol
Fusarium oxysporum
Transaldolase
Xylose fermentation

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10.1007/s12010-011-9191-5

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Fan, J. X., Yang, X. X., Song, J. Z., Huang, X. M., Cheng, Z. X., Yao, L., Juba, O. S., Liang, Q., Yang, Q., Odeph, M., Sun, Y., & Wang, Y. (2011). Heterologous expression of transaldolase gene tal from saccharomyces cerevisiae in fusarium oxysporum for enhanced bioethanol production. Applied Biochemistry and Biotechnology, 164(7), 1023-1036. https://doi.org/10.1007/s12010-011-9191-5

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title = "Heterologous expression of transaldolase gene tal from saccharomyces cerevisiae in fusarium oxysporum for enhanced bioethanol production",

abstract = "The filamentous fungus Fusarium oxysporum is known for its ability to ferment xylose-producing ethanol. However, efficiency of xylose utilization and ethanol yield was low. In this study, the transaldolase gene from Saccharomyces cerevisiae has been successfully expressed in F. oxysporum by an Agrobacterium tumefaciens-mediated transformation method. The enzymatic activity of the recombinant fungus (cs28pCAM-Sctal4) was 0.195 times higher than that of the wild-type strain (cs28). The recombinant strain also exhibited a 28.83\% increase in ethanol yield on xylose media compared to the parental strain. Enhanced ethanol production and a reduction in the biomass were observed during xylose fermentation. Ethanol yield from rice straw by simultaneous saccharification and fermentation with cs28pCAM-Sctal4 was 0.25 gg-1 of rice straw. The transgenic strain of F. oxysporum cs28pCAM-Sctal4 might therefore have potential applications in industrial bioenergy production.",

keywords = "Bioethanol, Fusarium oxysporum, Transaldolase, Xylose fermentation",

author = "Fan, \{Jin Xia\} and Yang, \{Xiao Xue\} and Song, \{Jin Zhu\} and Huang, \{Xiao Mei\} and Cheng, \{Zhong Xiang\} and Lin Yao and Juba, \{Olivia S.\} and Qing Liang and Qian Yang and Margaret Odeph and Yan Sun and Yun Wang",

year = "2011",

month = aug,

doi = "10.1007/s12010-011-9191-5",

language = "英语",

volume = "164",

pages = "1023--1036",

journal = "Applied Biochemistry and Biotechnology",

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Fan, JX, Yang, XX, Song, JZ, Huang, XM, Cheng, ZX, Yao, L, Juba, OS, Liang, Q, Yang, Q, Odeph, M, Sun, Y & Wang, Y 2011, 'Heterologous expression of transaldolase gene tal from saccharomyces cerevisiae in fusarium oxysporum for enhanced bioethanol production', Applied Biochemistry and Biotechnology, vol. 164, no. 7, pp. 1023-1036. https://doi.org/10.1007/s12010-011-9191-5

TY - JOUR

T1 - Heterologous expression of transaldolase gene tal from saccharomyces cerevisiae in fusarium oxysporum for enhanced bioethanol production

AU - Fan, Jin Xia

AU - Yang, Xiao Xue

AU - Song, Jin Zhu

AU - Huang, Xiao Mei

AU - Cheng, Zhong Xiang

AU - Yao, Lin

AU - Juba, Olivia S.

AU - Liang, Qing

AU - Yang, Qian

AU - Odeph, Margaret

AU - Sun, Yan

AU - Wang, Yun

PY - 2011/8

Y1 - 2011/8

N2 - The filamentous fungus Fusarium oxysporum is known for its ability to ferment xylose-producing ethanol. However, efficiency of xylose utilization and ethanol yield was low. In this study, the transaldolase gene from Saccharomyces cerevisiae has been successfully expressed in F. oxysporum by an Agrobacterium tumefaciens-mediated transformation method. The enzymatic activity of the recombinant fungus (cs28pCAM-Sctal4) was 0.195 times higher than that of the wild-type strain (cs28). The recombinant strain also exhibited a 28.83% increase in ethanol yield on xylose media compared to the parental strain. Enhanced ethanol production and a reduction in the biomass were observed during xylose fermentation. Ethanol yield from rice straw by simultaneous saccharification and fermentation with cs28pCAM-Sctal4 was 0.25 gg-1 of rice straw. The transgenic strain of F. oxysporum cs28pCAM-Sctal4 might therefore have potential applications in industrial bioenergy production.

AB - The filamentous fungus Fusarium oxysporum is known for its ability to ferment xylose-producing ethanol. However, efficiency of xylose utilization and ethanol yield was low. In this study, the transaldolase gene from Saccharomyces cerevisiae has been successfully expressed in F. oxysporum by an Agrobacterium tumefaciens-mediated transformation method. The enzymatic activity of the recombinant fungus (cs28pCAM-Sctal4) was 0.195 times higher than that of the wild-type strain (cs28). The recombinant strain also exhibited a 28.83% increase in ethanol yield on xylose media compared to the parental strain. Enhanced ethanol production and a reduction in the biomass were observed during xylose fermentation. Ethanol yield from rice straw by simultaneous saccharification and fermentation with cs28pCAM-Sctal4 was 0.25 gg-1 of rice straw. The transgenic strain of F. oxysporum cs28pCAM-Sctal4 might therefore have potential applications in industrial bioenergy production.

KW - Bioethanol

KW - Fusarium oxysporum

KW - Transaldolase

KW - Xylose fermentation

UR - https://www.scopus.com/pages/publications/79960101177

U2 - 10.1007/s12010-011-9191-5

DO - 10.1007/s12010-011-9191-5

M3 - 文章

C2 - 21394668

AN - SCOPUS:79960101177

SN - 0273-2289

VL - 164

SP - 1023

EP - 1036

JO - Applied Biochemistry and Biotechnology

JF - Applied Biochemistry and Biotechnology

IS - 7

ER -

Heterologous expression of transaldolase gene tal from saccharomyces cerevisiae in fusarium oxysporum for enhanced bioethanol production

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