Glycine betaine transport conditions of Lactobacillus delbrueckii subsp. bulgaricus in salt induced hyperosmotic stress

Xue Han; Huiying Wu; Peng Yu; Lijuan Zhang; Sainan Zhao; Lanwei Zhang

doi:10.1016/j.idairyj.2018.06.007

Glycine betaine transport conditions of Lactobacillus delbrueckii subsp. bulgaricus in salt induced hyperosmotic stress

Xue Han^*
, Huiying Wu
, Peng Yu
, Lijuan Zhang
, Sainan Zhao
, Lanwei Zhang

^*Corresponding author for this work

School of Chemistry and Chemical Engineering, Harbin Institute of Technology
Dairy Research Institute of Bright Dairy & Food Co. Ltd.
Ocean University of China

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

Glycine betaine (GB) is a naturally occurring osmolyte that helps cells adapt to osmotic stress. In this study, the osmoprotective role of GB in Lactobacillus delbrueckii subsp. bulgaricus 34.5 under salt stress was investigated. A concentration of 0.2 M NaCl provided sufficient osmotic constraint while allowing the cells to survive. Under conditions of salt stress, Lactobacillus bulgaricus 34.5 continued to proliferate in the presence of GB, and the optimum growth was seen at 1.2 mM GB. The optimum culture conditions for L. bulgaricus 34.5 were 37 °C and pH 6.0 for 24 h. In addition, intracellular GB content increased in response to salt stress, and the extent of GB transport in L. bulgaricus 34.5 under high salt concentrations depended on the culture conditions. In conclusion, GB played a protective role in alleviating the detrimental effect of salt on L. bulgaricus 34.5.

Original language	English
Pages (from-to)	21-26
Number of pages	6
Journal	International Dairy Journal
Volume	86
DOIs	https://doi.org/10.1016/j.idairyj.2018.06.007
State	Published - Nov 2018
Externally published	Yes

Access to Document

10.1016/j.idairyj.2018.06.007

Cite this

@article{3ca2b29db3844279be6995ff5008434d,

title = "Glycine betaine transport conditions of Lactobacillus delbrueckii subsp. bulgaricus in salt induced hyperosmotic stress",

abstract = "Glycine betaine (GB) is a naturally occurring osmolyte that helps cells adapt to osmotic stress. In this study, the osmoprotective role of GB in Lactobacillus delbrueckii subsp. bulgaricus 34.5 under salt stress was investigated. A concentration of 0.2 M NaCl provided sufficient osmotic constraint while allowing the cells to survive. Under conditions of salt stress, Lactobacillus bulgaricus 34.5 continued to proliferate in the presence of GB, and the optimum growth was seen at 1.2 mM GB. The optimum culture conditions for L. bulgaricus 34.5 were 37 °C and pH 6.0 for 24 h. In addition, intracellular GB content increased in response to salt stress, and the extent of GB transport in L. bulgaricus 34.5 under high salt concentrations depended on the culture conditions. In conclusion, GB played a protective role in alleviating the detrimental effect of salt on L. bulgaricus 34.5.",

author = "Xue Han and Huiying Wu and Peng Yu and Lijuan Zhang and Sainan Zhao and Lanwei Zhang",

note = "Publisher Copyright: {\textcopyright} 2018 Elsevier Ltd",

year = "2018",

month = nov,

doi = "10.1016/j.idairyj.2018.06.007",

language = "英语",

volume = "86",

pages = "21--26",

journal = "International Dairy Journal",

issn = "0958-6946",

publisher = "Elsevier B.V.",

}

TY - JOUR

T1 - Glycine betaine transport conditions of Lactobacillus delbrueckii subsp. bulgaricus in salt induced hyperosmotic stress

AU - Han, Xue

AU - Wu, Huiying

AU - Yu, Peng

AU - Zhang, Lijuan

AU - Zhao, Sainan

AU - Zhang, Lanwei

PY - 2018/11

Y1 - 2018/11

N2 - Glycine betaine (GB) is a naturally occurring osmolyte that helps cells adapt to osmotic stress. In this study, the osmoprotective role of GB in Lactobacillus delbrueckii subsp. bulgaricus 34.5 under salt stress was investigated. A concentration of 0.2 M NaCl provided sufficient osmotic constraint while allowing the cells to survive. Under conditions of salt stress, Lactobacillus bulgaricus 34.5 continued to proliferate in the presence of GB, and the optimum growth was seen at 1.2 mM GB. The optimum culture conditions for L. bulgaricus 34.5 were 37 °C and pH 6.0 for 24 h. In addition, intracellular GB content increased in response to salt stress, and the extent of GB transport in L. bulgaricus 34.5 under high salt concentrations depended on the culture conditions. In conclusion, GB played a protective role in alleviating the detrimental effect of salt on L. bulgaricus 34.5.

AB - Glycine betaine (GB) is a naturally occurring osmolyte that helps cells adapt to osmotic stress. In this study, the osmoprotective role of GB in Lactobacillus delbrueckii subsp. bulgaricus 34.5 under salt stress was investigated. A concentration of 0.2 M NaCl provided sufficient osmotic constraint while allowing the cells to survive. Under conditions of salt stress, Lactobacillus bulgaricus 34.5 continued to proliferate in the presence of GB, and the optimum growth was seen at 1.2 mM GB. The optimum culture conditions for L. bulgaricus 34.5 were 37 °C and pH 6.0 for 24 h. In addition, intracellular GB content increased in response to salt stress, and the extent of GB transport in L. bulgaricus 34.5 under high salt concentrations depended on the culture conditions. In conclusion, GB played a protective role in alleviating the detrimental effect of salt on L. bulgaricus 34.5.

UR - https://www.scopus.com/pages/publications/85049974577

U2 - 10.1016/j.idairyj.2018.06.007

DO - 10.1016/j.idairyj.2018.06.007

M3 - 文章

AN - SCOPUS:85049974577

SN - 0958-6946

VL - 86

SP - 21

EP - 26

JO - International Dairy Journal

JF - International Dairy Journal

ER -

Glycine betaine transport conditions of Lactobacillus delbrueckii subsp. bulgaricus in salt induced hyperosmotic stress

Abstract

Access to Document

Other files and links

Fingerprint

Cite this