Fungus-sourced filament-array anode facilitates Geobacter enrichment and promotes anodic bio-capacitance improvement for efficient power generation in microbial fuel cells

Microbial fuel cells (MFC) are emerging as new generation eco-friendly technology for the superiorities of energy harvest and simultaneous wastewater treatment. However, the power generation performance was strongly restricted by the material/biofilm electron transfer rate. In this research, the fungus-sourced electrode with filament-array structure was firstly proposed and prepared by one-step carbonization method. After 2 h pyrolysis, the functional groups containing N and O elements highly remained in the as-prepared material, which was beneficial to the electron transfer for the current generation. The lowest electron transfer resistance was obtained at 2.2 Ω, which showed a great reduction that compared with graphite sheet anode. With filament-array structure, the lowest mass diffusion resistance was obtained at 26.9 Ω for anodic oxidation reaction, which also supported the highest current generation performance. In addition, the relative abundance of typical electrochemical bacterium Geobacter was highly improved to 45.5% with an extraordinary electroactive biofilm loading of about 1203 ± 256 μg cm⁻². More importantly, the high biocatalytic activity biofilm supported a remarkably observed bio-capacitance of about 1.14 F in 3DF_fv anode, which exhibited the highest power density in 3.5 ± 0.2 W m⁻². In addition, the fungus-sourced material was one kind of economical and readily available material. Overall, this work provided one efficient strategy for electrode preparation and higher power generation in MFCs, which would reduce the capital cost and improve the efficiency in further applications of MFCs.