Functional bacterial and archaeal dynamics dictated by pH stress during sugar refinery wastewater in a UASB

The operation performance and microbial mechanisms by pH stress were investigated during anaerobic digestion of sugar refinery wastewater in a upflow anaerobic sludge blanket (UASB) reactor to clarify correlations between pH stress, microbial community and process efficiency. Results showed that the COD removal and methane yield were respectively reduced by 24.8% and 25.3% as pH decreased to 5.0. pH decrease resulted in the composition of dominant fermentative acidogenic bacteria was changed to Butyricicoccus, Lactococcus, Brooklawnia, Armatimonadetes_gp2 and Megasphaera from Prevotella, Streptococcus, Acidaminococcus and Megasphaera, causing an increase in propionate production. In addition, the growth of propionate-oxidizing bacteria was also inhibited at pH 5.0, leading the propionate was accumulated, and then reduced the process efficiency. Methane was mainly produced through acetate cleavage by Methanosaeta during the whole operational period of UASB. pH decrease blocked the metabolic balance and community structure between different trophic groups, resulting in the decrease in reactor performance.