TY - GEN
T1 - Force characterization of live cells in automated transportation with robot-tweezers manipulation system
AU - Wu, Yanhua
AU - Chen, Haoyao
AU - Sun, Dong
AU - Huang, Wenhao
PY - 2010
Y1 - 2010
N2 - Optical trap provides a new and unique tool for manipulation in micro/nano scale and have wide applications in biological fields. Increasing demands for both accuracy and efficiency in cell manipulation highlight the need for automation process that integrates robotics and optical tweezers technologies. A clear understanding of the forces exerted on cells, i.e., trapping and viscous drag forces, is essential to enable automated manipulation using optical tweezers. In this paper, we propose a method to characterize the forces exerted on the live cell moved by optical trap, where the cell is bound to a micro polystyrene bead. Experiment of the cell falling to the focus of the optical trap is performed to show that the proposed force calibration method exhibit better accuracy than the traditional ray-optic numerical method based on the transparent-bead model. Further, automated transportation of the yeast cell is performed to demonstrate the effectiveness in applying the proposed force characterization approach to automated cell manipulation.
AB - Optical trap provides a new and unique tool for manipulation in micro/nano scale and have wide applications in biological fields. Increasing demands for both accuracy and efficiency in cell manipulation highlight the need for automation process that integrates robotics and optical tweezers technologies. A clear understanding of the forces exerted on cells, i.e., trapping and viscous drag forces, is essential to enable automated manipulation using optical tweezers. In this paper, we propose a method to characterize the forces exerted on the live cell moved by optical trap, where the cell is bound to a micro polystyrene bead. Experiment of the cell falling to the focus of the optical trap is performed to show that the proposed force calibration method exhibit better accuracy than the traditional ray-optic numerical method based on the transparent-bead model. Further, automated transportation of the yeast cell is performed to demonstrate the effectiveness in applying the proposed force characterization approach to automated cell manipulation.
UR - https://www.scopus.com/pages/publications/78649263808
U2 - 10.1109/ICMA.2010.5588938
DO - 10.1109/ICMA.2010.5588938
M3 - 会议稿件
AN - SCOPUS:78649263808
SN - 9781424451418
T3 - 2010 IEEE International Conference on Mechatronics and Automation, ICMA 2010
SP - 1913
EP - 1918
BT - 2010 IEEE International Conference on Mechatronics and Automation, ICMA 2010
T2 - 2010 IEEE International Conference on Mechatronics and Automation, ICMA 2010
Y2 - 4 August 2010 through 7 August 2010
ER -