TY - GEN
T1 - Fermentation condition optimization of bio-demulsifying bacteria ( a Bacillus sp.) using response surface methodology
AU - Li, Xu
AU - Liu, Chang
AU - Ma, Fang
AU - Yang, Jixian
AU - Wang, Jinna
PY - 2011
Y1 - 2011
N2 - This work investigated the effect of physiochemical conditions of fermentation on the de-emulsification capability of biological de-emulsifier production by a demulsifying strain (a Bacillus sp. isolated by our laboratory from oil contaminated soil). A quadratic orthogonal regressive rotation design was used to establish an effective statistical model for evaluation the combined effects of the incubation time(A), culturing temperature(B), glucose concentration(C) in substrate, inoculum age(D) and shaker agitation(E). The results of analysis of variance (ANOVA) of face-centered central composite design (FCCCD) showed that the adequately high R2 value of 0.976 and F-value of 30.69 indicated the statistical significance of the quadratic model which there were seven significant model terms (Prob>F<0.0500), namely B, C, E, AC, AE, C2, E2. De-emulsification capability was enhanced when the culturing temperature as a main influence factor was more than 35°C. The three-dimensional response surface plot and contour plot present the maximum demulsifying ratio was reached when the glucose concentration was at 8.5g/L∼16.25g/L, the incubation time at the range of 15h∼21h, the shaker agitation exceeded 150r/min and the inoculum age was at 16.5h∼25.5h.
AB - This work investigated the effect of physiochemical conditions of fermentation on the de-emulsification capability of biological de-emulsifier production by a demulsifying strain (a Bacillus sp. isolated by our laboratory from oil contaminated soil). A quadratic orthogonal regressive rotation design was used to establish an effective statistical model for evaluation the combined effects of the incubation time(A), culturing temperature(B), glucose concentration(C) in substrate, inoculum age(D) and shaker agitation(E). The results of analysis of variance (ANOVA) of face-centered central composite design (FCCCD) showed that the adequately high R2 value of 0.976 and F-value of 30.69 indicated the statistical significance of the quadratic model which there were seven significant model terms (Prob>F<0.0500), namely B, C, E, AC, AE, C2, E2. De-emulsification capability was enhanced when the culturing temperature as a main influence factor was more than 35°C. The three-dimensional response surface plot and contour plot present the maximum demulsifying ratio was reached when the glucose concentration was at 8.5g/L∼16.25g/L, the incubation time at the range of 15h∼21h, the shaker agitation exceeded 150r/min and the inoculum age was at 16.5h∼25.5h.
KW - Demulsifying Bacteria
KW - Demulsifying ratio
KW - Face-centered central composite design
KW - Fermentation condition
UR - https://www.scopus.com/pages/publications/79955854650
U2 - 10.1109/CDCIEM.2011.214
DO - 10.1109/CDCIEM.2011.214
M3 - 会议稿件
AN - SCOPUS:79955854650
SN - 9780769543505
T3 - Proceedings - International Conference on Computer Distributed Control and Intelligent Environmental Monitoring, CDCIEM 2011
SP - 433
EP - 437
BT - Proceedings - International Conference on Computer Distributed Control and Intelligent Environmental Monitoring, CDCIEM 2011
T2 - 2011 International Conference on Computer Distributed Control and Intelligent Environmental Monitoring, CDCIEM 2011
Y2 - 19 February 2011 through 20 February 2011
ER -