Fabrication of protein nanotubes based on layer-by-layer assembly

Ying Tian; Qiang He; Yue Cui; Junbai Li

doi:10.1021/bm060412l

Fabrication of protein nanotubes based on layer-by-layer assembly

Ying Tian
, Qiang He
, Yue Cui
, Junbai Li^*

^*Corresponding author for this work

CAS - Institute of Chemistry

Research output: Contribution to journal › Article › peer-review

91 Scopus citations

Abstract

Nanotubes of cytochrome C (cyto-c) with glutaraldehyde (GA) or PSS based on the layer-by-layer (LbL) assembly through covalent binding and electrostatic adsorption have been fabricated. The combination of the template method and the LbL method for fabrication of nanotubes exhibits low cost, simplicity, and versatility. The tubular morphology of the assembled glutaraldehyde and cytochrome C film was demonstrated by scanning electron microscopy (SEM) and transmission electron microscopy (TEM) measurements. The components of the tubes were determined by energy-dispersive X- ray spectra (EDAX). It is found that the assembled tubes keep the proteins' biochemical activity and electronic activity by cyclic voltammograms. The measurements of ultraviolet spectra and circular dichroism (CD) on the assembled nanotubes confirmed the cyto-c existence in the tubes.

Original language	English
Pages (from-to)	2539-2542
Number of pages	4
Journal	Biomacromolecules
Volume	7
Issue number	9
DOIs	https://doi.org/10.1021/bm060412l
State	Published - Sep 2006
Externally published	Yes

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10.1021/bm060412l

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title = "Fabrication of protein nanotubes based on layer-by-layer assembly",

abstract = "Nanotubes of cytochrome C (cyto-c) with glutaraldehyde (GA) or PSS based on the layer-by-layer (LbL) assembly through covalent binding and electrostatic adsorption have been fabricated. The combination of the template method and the LbL method for fabrication of nanotubes exhibits low cost, simplicity, and versatility. The tubular morphology of the assembled glutaraldehyde and cytochrome C film was demonstrated by scanning electron microscopy (SEM) and transmission electron microscopy (TEM) measurements. The components of the tubes were determined by energy-dispersive X- ray spectra (EDAX). It is found that the assembled tubes keep the proteins' biochemical activity and electronic activity by cyclic voltammograms. The measurements of ultraviolet spectra and circular dichroism (CD) on the assembled nanotubes confirmed the cyto-c existence in the tubes.",

author = "Ying Tian and Qiang He and Yue Cui and Junbai Li",

year = "2006",

month = sep,

doi = "10.1021/bm060412l",

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T1 - Fabrication of protein nanotubes based on layer-by-layer assembly

AU - Tian, Ying

AU - He, Qiang

AU - Cui, Yue

AU - Li, Junbai

PY - 2006/9

Y1 - 2006/9

N2 - Nanotubes of cytochrome C (cyto-c) with glutaraldehyde (GA) or PSS based on the layer-by-layer (LbL) assembly through covalent binding and electrostatic adsorption have been fabricated. The combination of the template method and the LbL method for fabrication of nanotubes exhibits low cost, simplicity, and versatility. The tubular morphology of the assembled glutaraldehyde and cytochrome C film was demonstrated by scanning electron microscopy (SEM) and transmission electron microscopy (TEM) measurements. The components of the tubes were determined by energy-dispersive X- ray spectra (EDAX). It is found that the assembled tubes keep the proteins' biochemical activity and electronic activity by cyclic voltammograms. The measurements of ultraviolet spectra and circular dichroism (CD) on the assembled nanotubes confirmed the cyto-c existence in the tubes.

AB - Nanotubes of cytochrome C (cyto-c) with glutaraldehyde (GA) or PSS based on the layer-by-layer (LbL) assembly through covalent binding and electrostatic adsorption have been fabricated. The combination of the template method and the LbL method for fabrication of nanotubes exhibits low cost, simplicity, and versatility. The tubular morphology of the assembled glutaraldehyde and cytochrome C film was demonstrated by scanning electron microscopy (SEM) and transmission electron microscopy (TEM) measurements. The components of the tubes were determined by energy-dispersive X- ray spectra (EDAX). It is found that the assembled tubes keep the proteins' biochemical activity and electronic activity by cyclic voltammograms. The measurements of ultraviolet spectra and circular dichroism (CD) on the assembled nanotubes confirmed the cyto-c existence in the tubes.

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DO - 10.1021/bm060412l

M3 - 文章

C2 - 16961315

AN - SCOPUS:33749550381

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SP - 2539

EP - 2542

JO - Biomacromolecules

JF - Biomacromolecules

IS - 9

ER -

Fabrication of protein nanotubes based on layer-by-layer assembly

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