Abstract
In this paper, we describe a simple method for fabrication of high quality poly(dimethylsiloxane) (PDMS)/glass microchip by twofold replica molding of PDMS. This technique first served to transfer the negative microchannels from the glass template to the PDMS substrate as a master, and then this PDMS master with positive microchannels was used to replicate the PDMS replica with negative microchannels. Finally, the PDMS replica was bound to a glass sheet by UV radiation. The fabricated microchips were successfully applied for the detection of C677T mutation from the human methylenetetrahydrofolate reductase gene.
| Original language | English |
|---|---|
| Pages (from-to) | 2390-2394 |
| Number of pages | 5 |
| Journal | Journal of Separation Science |
| Volume | 29 |
| Issue number | 15 |
| DOIs | |
| State | Published - Oct 2006 |
| Externally published | Yes |
Keywords
- Fabrication
- Genetic analysis
- Microchip
- Plly(dimethylsiloxane)
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