@inproceedings{655e607578b34c608bb0e28fd28d34c4,
title = "Extraction of a novel de-emulsifier by organic solvent",
abstract = "Isolation and identification of key components of the crude de-emulsifier produced by Bacillus mojavensis was studied. Organic solvent precipitation method was used to extract active ingredients from cell free broth. Crude products extracted by different solvent showed diverse de-emulsification activity. 84\% and 73\% of the de-emulsifier in cell free broth could be extracted successfully by using isopropanol and ethanol respectively. UV analysis demonstrated the presence of protein in the extraction solution. Protein concentration of extracted products solution related with its de-emulsification activity. The maximum protein concentration (0.241mg/ml) and maximum oil drain rate were found in isopropanol extracted product. While the acetone extraction showed lowest protein concentration and lowest de-emulsification activity. SDS-PAGE and IR spectroscopy results confrimed that protein of microbial metabolites played important roles in de-emulsification.",
keywords = "De-emulsifier, Extraction, Oil drain rate, Protein",
author = "Xu Yang and Ma Fang and Li Xu and Dai Yang and Hou Ning",
year = "2009",
doi = "10.1109/ICEET.2009.375",
language = "英语",
isbn = "9780769538198",
series = "2009 International Conference on Energy and Environment Technology, ICEET 2009",
pages = "565--568",
booktitle = "2009 International Conference on Energy and Environment Technology, ICEET 2009",
note = "2009 International Conference on Energy and Environment Technology, ICEET 2009 ; Conference date: 16-10-2009 Through 18-10-2009",
}