Extracellular biological organic matters in microbial fuel cell using sewage sludge as fuel

The microbial fuel cell (MFC) was applied to produce electricity using sewage sludge as a fuel. The extracellular biological organic matter (EBOM) of sludge, before and after MFC operation, was extracted using ammonium hydroxide whose hydrophobicity was characterized with XAD resin fractionation technique. Following MFC operation, the hydrophilic fraction (HPI) of EBOM was enriched (48.0%-64.5%), the hydrophobic acid (HPO-A) fraction was reduced (32.0%-14.5%), and the dissolved organic carbon (DOC) was removed by 36.8% and the sludge aromaticity decreased by 65.7%. The fluorescence tests indicated that the MFC removed the aromatic proteins-like and soluble microbial byproduct-like materials in sludge. Fourier-transform infrared (FT-IR) results showed that the aliphatic (C-H related) components, hydrocarbon and carbohydrate were easily hydrolyzed and biodegraded in the studied MFC.