Expression of the chitinase gene from Trichoderma aureoviride in Saccharomyces cerevisiae

Song Jinzhu; Yang Qian; Liu Beidong; Chen Dianfu

doi:10.1007/s00253-005-1957-7

Expression of the chitinase gene from Trichoderma aureoviride in Saccharomyces cerevisiae

Song Jinzhu^*
, Yang Qian
, Liu Beidong
, Chen Dianfu

^*Corresponding author for this work

Faculty of Life Sciences and Medicine

Harbin Institute of Technology

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

Chitinase gene ech42 was obtained from Trichoderma aureoviride M and amplified by PCR. The isolated DNA of ech42 was then sequenced. The results showed that the open reading frame of ech42 was 1,447 bp long, encoding 421 amino acids. Three introns were found in the sequence. The cloning vector pMD18-T and an E. coli DH5α host were used to yield clones as E. coli DH5α/ech42. The ech42 gene was integrated into the genomic DNA of pYES2 by insertion into a single site for recombination, yielding the recombinant pYES2/ech42. Chitinase expressed by pYES2/ech42 was induced by galactose (maximal activity 0.50 units ml^-1) and was produced in fermentation liquid cultured for 36 h.

Original language	English
Pages (from-to)	39-43
Number of pages	5
Journal	Applied Microbiology and Biotechnology
Volume	69
Issue number	1
DOIs	https://doi.org/10.1007/s00253-005-1957-7
State	Published - Nov 2005

Access to Document

10.1007/s00253-005-1957-7

Cite this

@article{fe9290e766c14a2dbe8beb847c1f0794,

title = "Expression of the chitinase gene from Trichoderma aureoviride in Saccharomyces cerevisiae",

abstract = "Chitinase gene ech42 was obtained from Trichoderma aureoviride M and amplified by PCR. The isolated DNA of ech42 was then sequenced. The results showed that the open reading frame of ech42 was 1,447 bp long, encoding 421 amino acids. Three introns were found in the sequence. The cloning vector pMD18-T and an E. coli DH5α host were used to yield clones as E. coli DH5α/ech42. The ech42 gene was integrated into the genomic DNA of pYES2 by insertion into a single site for recombination, yielding the recombinant pYES2/ech42. Chitinase expressed by pYES2/ech42 was induced by galactose (maximal activity 0.50 units ml-1) and was produced in fermentation liquid cultured for 36 h.",

author = "Song Jinzhu and Yang Qian and Liu Beidong and Chen Dianfu",

year = "2005",

month = nov,

doi = "10.1007/s00253-005-1957-7",

language = "英语",

volume = "69",

pages = "39--43",

journal = "Applied Microbiology and Biotechnology",

issn = "0175-7598",

publisher = "Springer Science and Business Media Deutschland GmbH",

number = "1",

}

TY - JOUR

T1 - Expression of the chitinase gene from Trichoderma aureoviride in Saccharomyces cerevisiae

AU - Jinzhu, Song

AU - Qian, Yang

AU - Beidong, Liu

AU - Dianfu, Chen

PY - 2005/11

Y1 - 2005/11

N2 - Chitinase gene ech42 was obtained from Trichoderma aureoviride M and amplified by PCR. The isolated DNA of ech42 was then sequenced. The results showed that the open reading frame of ech42 was 1,447 bp long, encoding 421 amino acids. Three introns were found in the sequence. The cloning vector pMD18-T and an E. coli DH5α host were used to yield clones as E. coli DH5α/ech42. The ech42 gene was integrated into the genomic DNA of pYES2 by insertion into a single site for recombination, yielding the recombinant pYES2/ech42. Chitinase expressed by pYES2/ech42 was induced by galactose (maximal activity 0.50 units ml-1) and was produced in fermentation liquid cultured for 36 h.

AB - Chitinase gene ech42 was obtained from Trichoderma aureoviride M and amplified by PCR. The isolated DNA of ech42 was then sequenced. The results showed that the open reading frame of ech42 was 1,447 bp long, encoding 421 amino acids. Three introns were found in the sequence. The cloning vector pMD18-T and an E. coli DH5α host were used to yield clones as E. coli DH5α/ech42. The ech42 gene was integrated into the genomic DNA of pYES2 by insertion into a single site for recombination, yielding the recombinant pYES2/ech42. Chitinase expressed by pYES2/ech42 was induced by galactose (maximal activity 0.50 units ml-1) and was produced in fermentation liquid cultured for 36 h.

UR - https://www.scopus.com/pages/publications/28344455221

U2 - 10.1007/s00253-005-1957-7

DO - 10.1007/s00253-005-1957-7

M3 - 文章

C2 - 15809824

AN - SCOPUS:28344455221

SN - 0175-7598

VL - 69

SP - 39

EP - 43

JO - Applied Microbiology and Biotechnology

JF - Applied Microbiology and Biotechnology

IS - 1

ER -

Expression of the chitinase gene from Trichoderma aureoviride in Saccharomyces cerevisiae

Abstract

Access to Document

Other files and links

Fingerprint

Cite this