Expression of histone deacetylase 9 in bone marrow mesenchymal stem cells during senescence

Yuanbo Zhan; Xinpeng Liu; Wenxia Xu; Nan Miao; Sen Mu; Ruimin Zhang; Ying Li

doi:10.12307/2022.754

Expression of histone deacetylase 9 in bone marrow mesenchymal stem cells during senescence

Yuanbo Zhan
, Xinpeng Liu
, Wenxia Xu
, Nan Miao
, Sen Mu
, Ruimin Zhang
, Ying Li^*

^*Corresponding author for this work

The Second Affiliated Hospital of Harbin Medical University
Heilongjiang Provincial Key Laboratory of Hard Tissue Development and Regeneration

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

BACKGROUND: Bone marrow mesenchymal stem cells have aging phenomenon during in vitro culture, but the mechanism is still unclear. Histone deacetylases can deacetylate histones and make the chromatin condense, further shutting down genes. OBJECTIVE: To investigate the expression pattern of histone deacetylase 9 in bone marrow mesenchymal stem cells during senescence. METHODS: The expression of histone deacetylases in bone marrow mesenchymal stem cells was analyzed by bioinformatics. Bone marrow mesenchymal stem cells were cultured by in vitro subculture. The senescence status of bone marrow mesenchymal stem cells was detected by β-galactosidase staining and qRT-PCR. The expression pattern of histone deacetylase 9 was detected by qRT-PCR, western blot assay, and immunofluorescence. RESULTS AND CONCLUSION: (1) Data mining and bioinformatics analysis showed that only histone deacetylase 9 expression was up-regulated among all histone deacetylase subtypes during bone marrow mesenchymal stem cell senescence. (2) Bone marrow mesenchymal stem cells presented obvious senescence at passage 10; for example, β-galactosidase-positive cells were the most obvious, and the expression of senescence-related genes was up-regulated. (3) The expression of histone deacetylase 9 was up-regulated and consistent with the data mining results. Histone deacetylase 9 was expressed in both the nucleus and cytoplasm of young bone marrow mesenchymal stem cells, but the expression in the nucleus was lower than that in the nucleus of senescence bone marrow mesenchymal stem cells. The expression of histone deacetylase 9 was almost not expressed in the cytoplasm of senescence bone marrow mesenchymal stem cells, but increased in the nucleus. (4) The results have shown that histone deacetylase 9 plays an important role in the senescence process of bone marrow mesenchymal stem cells, but the aging phenotype and molecular mechanism of histone deacetylase 9 need to be further studied.

Original language	English
Pages (from-to)	4762-4766
Number of pages	5
Journal	Chinese Journal of Tissue Engineering Research
Volume	26
Issue number	30
DOIs	https://doi.org/10.12307/2022.754
State	Published - 28 Oct 2022
Externally published	Yes

Keywords

bone marrow mesenchymal stem cells
cellular senescence
histone deacetylase 9
natural aging
stem cells

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10.12307/2022.754

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@article{49739163e4f849e9980a0ba7a7f26ffb,

title = "Expression of histone deacetylase 9 in bone marrow mesenchymal stem cells during senescence",

abstract = "BACKGROUND: Bone marrow mesenchymal stem cells have aging phenomenon during in vitro culture, but the mechanism is still unclear. Histone deacetylases can deacetylate histones and make the chromatin condense, further shutting down genes. OBJECTIVE: To investigate the expression pattern of histone deacetylase 9 in bone marrow mesenchymal stem cells during senescence. METHODS: The expression of histone deacetylases in bone marrow mesenchymal stem cells was analyzed by bioinformatics. Bone marrow mesenchymal stem cells were cultured by in vitro subculture. The senescence status of bone marrow mesenchymal stem cells was detected by β-galactosidase staining and qRT-PCR. The expression pattern of histone deacetylase 9 was detected by qRT-PCR, western blot assay, and immunofluorescence. RESULTS AND CONCLUSION: (1) Data mining and bioinformatics analysis showed that only histone deacetylase 9 expression was up-regulated among all histone deacetylase subtypes during bone marrow mesenchymal stem cell senescence. (2) Bone marrow mesenchymal stem cells presented obvious senescence at passage 10; for example, β-galactosidase-positive cells were the most obvious, and the expression of senescence-related genes was up-regulated. (3) The expression of histone deacetylase 9 was up-regulated and consistent with the data mining results. Histone deacetylase 9 was expressed in both the nucleus and cytoplasm of young bone marrow mesenchymal stem cells, but the expression in the nucleus was lower than that in the nucleus of senescence bone marrow mesenchymal stem cells. The expression of histone deacetylase 9 was almost not expressed in the cytoplasm of senescence bone marrow mesenchymal stem cells, but increased in the nucleus. (4) The results have shown that histone deacetylase 9 plays an important role in the senescence process of bone marrow mesenchymal stem cells, but the aging phenotype and molecular mechanism of histone deacetylase 9 need to be further studied.",

keywords = "bone marrow mesenchymal stem cells, cellular senescence, histone deacetylase 9, natural aging, stem cells",

author = "Yuanbo Zhan and Xinpeng Liu and Wenxia Xu and Nan Miao and Sen Mu and Ruimin Zhang and Ying Li",

year = "2022",

month = oct,

day = "28",

doi = "10.12307/2022.754",

language = "英语",

volume = "26",

pages = "4762--4766",

journal = "Chinese Journal of Tissue Engineering Research",

issn = "2095-4344",

publisher = "Publishing House of Chinese Journal of Tissue Engineering Research",

number = "30",

}

TY - JOUR

T1 - Expression of histone deacetylase 9 in bone marrow mesenchymal stem cells during senescence

AU - Zhan, Yuanbo

AU - Liu, Xinpeng

AU - Xu, Wenxia

AU - Miao, Nan

AU - Mu, Sen

AU - Zhang, Ruimin

AU - Li, Ying

PY - 2022/10/28

Y1 - 2022/10/28

N2 - BACKGROUND: Bone marrow mesenchymal stem cells have aging phenomenon during in vitro culture, but the mechanism is still unclear. Histone deacetylases can deacetylate histones and make the chromatin condense, further shutting down genes. OBJECTIVE: To investigate the expression pattern of histone deacetylase 9 in bone marrow mesenchymal stem cells during senescence. METHODS: The expression of histone deacetylases in bone marrow mesenchymal stem cells was analyzed by bioinformatics. Bone marrow mesenchymal stem cells were cultured by in vitro subculture. The senescence status of bone marrow mesenchymal stem cells was detected by β-galactosidase staining and qRT-PCR. The expression pattern of histone deacetylase 9 was detected by qRT-PCR, western blot assay, and immunofluorescence. RESULTS AND CONCLUSION: (1) Data mining and bioinformatics analysis showed that only histone deacetylase 9 expression was up-regulated among all histone deacetylase subtypes during bone marrow mesenchymal stem cell senescence. (2) Bone marrow mesenchymal stem cells presented obvious senescence at passage 10; for example, β-galactosidase-positive cells were the most obvious, and the expression of senescence-related genes was up-regulated. (3) The expression of histone deacetylase 9 was up-regulated and consistent with the data mining results. Histone deacetylase 9 was expressed in both the nucleus and cytoplasm of young bone marrow mesenchymal stem cells, but the expression in the nucleus was lower than that in the nucleus of senescence bone marrow mesenchymal stem cells. The expression of histone deacetylase 9 was almost not expressed in the cytoplasm of senescence bone marrow mesenchymal stem cells, but increased in the nucleus. (4) The results have shown that histone deacetylase 9 plays an important role in the senescence process of bone marrow mesenchymal stem cells, but the aging phenotype and molecular mechanism of histone deacetylase 9 need to be further studied.

AB - BACKGROUND: Bone marrow mesenchymal stem cells have aging phenomenon during in vitro culture, but the mechanism is still unclear. Histone deacetylases can deacetylate histones and make the chromatin condense, further shutting down genes. OBJECTIVE: To investigate the expression pattern of histone deacetylase 9 in bone marrow mesenchymal stem cells during senescence. METHODS: The expression of histone deacetylases in bone marrow mesenchymal stem cells was analyzed by bioinformatics. Bone marrow mesenchymal stem cells were cultured by in vitro subculture. The senescence status of bone marrow mesenchymal stem cells was detected by β-galactosidase staining and qRT-PCR. The expression pattern of histone deacetylase 9 was detected by qRT-PCR, western blot assay, and immunofluorescence. RESULTS AND CONCLUSION: (1) Data mining and bioinformatics analysis showed that only histone deacetylase 9 expression was up-regulated among all histone deacetylase subtypes during bone marrow mesenchymal stem cell senescence. (2) Bone marrow mesenchymal stem cells presented obvious senescence at passage 10; for example, β-galactosidase-positive cells were the most obvious, and the expression of senescence-related genes was up-regulated. (3) The expression of histone deacetylase 9 was up-regulated and consistent with the data mining results. Histone deacetylase 9 was expressed in both the nucleus and cytoplasm of young bone marrow mesenchymal stem cells, but the expression in the nucleus was lower than that in the nucleus of senescence bone marrow mesenchymal stem cells. The expression of histone deacetylase 9 was almost not expressed in the cytoplasm of senescence bone marrow mesenchymal stem cells, but increased in the nucleus. (4) The results have shown that histone deacetylase 9 plays an important role in the senescence process of bone marrow mesenchymal stem cells, but the aging phenotype and molecular mechanism of histone deacetylase 9 need to be further studied.

KW - bone marrow mesenchymal stem cells

KW - cellular senescence

KW - histone deacetylase 9

KW - natural aging

KW - stem cells

UR - https://www.scopus.com/pages/publications/85128706626

U2 - 10.12307/2022.754

DO - 10.12307/2022.754

M3 - 文章

AN - SCOPUS:85128706626

SN - 2095-4344

VL - 26

SP - 4762

EP - 4766

JO - Chinese Journal of Tissue Engineering Research

JF - Chinese Journal of Tissue Engineering Research

IS - 30

ER -

Expression of histone deacetylase 9 in bone marrow mesenchymal stem cells during senescence

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