Expression of CspA and GST by an Antarctic psychrophilic bacterium Colwellia sp. NJ341 at near-freezing temperature

Quan Fu Wang; Jin Lai Miao; Yan Hua Hou; Yu Ding; Guang You Li

doi:10.1007/s11274-005-9010-7

Expression of CspA and GST by an Antarctic psychrophilic bacterium Colwellia sp. NJ341 at near-freezing temperature

Quan Fu Wang
, Jin Lai Miao^*
, Yan Hua Hou
, Yu Ding
, Guang You Li

^*Corresponding author for this work

Ocean University of China
Ministry of Natural Resources of the People's Republic of China
Harbin Institute of Technology Weihai

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

A psychrotrophic bacterium Colwellia sp. NJ341 from Antarctic sea ice could grow at -5 and 22°C, and the extent of cellular protein content and growth were greater at low temperatures (0-10°C) than at higher temperatures. SDS-PAGE analysis demonstrated the presence of a 7 kDa cold-shock protein. The further result of two-dimensional electrophoresis (2-DE) showed that two proteins a and c were newly synthesized at near-freezing temperatures. With matrix-assisted laser desorption/ionization mass spectrometry (MALDI-TOF MS) analysis, proteins a and c were identified as glutathione S-transferase (GST) and cold-shock protein A (CspA), respectively, which were involved in cold-adaptation at near-freezing temperature in an Antarctic psychrophilic bacterium Colwellia sp. NJ341.

Original language	English
Pages (from-to)	311-316
Number of pages	6
Journal	World Journal of Microbiology and Biotechnology
Volume	22
Issue number	4
DOIs	https://doi.org/10.1007/s11274-005-9010-7
State	Published - Apr 2006
Externally published	Yes

Keywords

Cold-adaptation
CspA
GST
Near-freezing temperature
Psychrophilic bacterium

Access to Document

10.1007/s11274-005-9010-7

Cite this

@article{0f2cdbeabbab4052ac732aec06cb02d7,

title = "Expression of CspA and GST by an Antarctic psychrophilic bacterium Colwellia sp. NJ341 at near-freezing temperature",

abstract = "A psychrotrophic bacterium Colwellia sp. NJ341 from Antarctic sea ice could grow at -5 and 22°C, and the extent of cellular protein content and growth were greater at low temperatures (0-10°C) than at higher temperatures. SDS-PAGE analysis demonstrated the presence of a 7 kDa cold-shock protein. The further result of two-dimensional electrophoresis (2-DE) showed that two proteins a and c were newly synthesized at near-freezing temperatures. With matrix-assisted laser desorption/ionization mass spectrometry (MALDI-TOF MS) analysis, proteins a and c were identified as glutathione S-transferase (GST) and cold-shock protein A (CspA), respectively, which were involved in cold-adaptation at near-freezing temperature in an Antarctic psychrophilic bacterium Colwellia sp. NJ341.",

keywords = "Cold-adaptation, CspA, GST, Near-freezing temperature, Psychrophilic bacterium",

author = "Wang, \{Quan Fu\} and Miao, \{Jin Lai\} and Hou, \{Yan Hua\} and Yu Ding and Li, \{Guang You\}",

year = "2006",

month = apr,

doi = "10.1007/s11274-005-9010-7",

language = "英语",

volume = "22",

pages = "311--316",

journal = "World Journal of Microbiology and Biotechnology",

issn = "0959-3993",

publisher = "Springer Science and Business Media B.V.",

number = "4",

}

TY - JOUR

T1 - Expression of CspA and GST by an Antarctic psychrophilic bacterium Colwellia sp. NJ341 at near-freezing temperature

AU - Wang, Quan Fu

AU - Miao, Jin Lai

AU - Hou, Yan Hua

AU - Ding, Yu

AU - Li, Guang You

PY - 2006/4

Y1 - 2006/4

N2 - A psychrotrophic bacterium Colwellia sp. NJ341 from Antarctic sea ice could grow at -5 and 22°C, and the extent of cellular protein content and growth were greater at low temperatures (0-10°C) than at higher temperatures. SDS-PAGE analysis demonstrated the presence of a 7 kDa cold-shock protein. The further result of two-dimensional electrophoresis (2-DE) showed that two proteins a and c were newly synthesized at near-freezing temperatures. With matrix-assisted laser desorption/ionization mass spectrometry (MALDI-TOF MS) analysis, proteins a and c were identified as glutathione S-transferase (GST) and cold-shock protein A (CspA), respectively, which were involved in cold-adaptation at near-freezing temperature in an Antarctic psychrophilic bacterium Colwellia sp. NJ341.

AB - A psychrotrophic bacterium Colwellia sp. NJ341 from Antarctic sea ice could grow at -5 and 22°C, and the extent of cellular protein content and growth were greater at low temperatures (0-10°C) than at higher temperatures. SDS-PAGE analysis demonstrated the presence of a 7 kDa cold-shock protein. The further result of two-dimensional electrophoresis (2-DE) showed that two proteins a and c were newly synthesized at near-freezing temperatures. With matrix-assisted laser desorption/ionization mass spectrometry (MALDI-TOF MS) analysis, proteins a and c were identified as glutathione S-transferase (GST) and cold-shock protein A (CspA), respectively, which were involved in cold-adaptation at near-freezing temperature in an Antarctic psychrophilic bacterium Colwellia sp. NJ341.

KW - Cold-adaptation

KW - CspA

KW - GST

KW - Near-freezing temperature

KW - Psychrophilic bacterium

UR - https://www.scopus.com/pages/publications/33646438077

U2 - 10.1007/s11274-005-9010-7

DO - 10.1007/s11274-005-9010-7

M3 - 文章

AN - SCOPUS:33646438077

SN - 0959-3993

VL - 22

SP - 311

EP - 316

JO - World Journal of Microbiology and Biotechnology

JF - World Journal of Microbiology and Biotechnology

IS - 4

ER -

Expression of CspA and GST by an Antarctic psychrophilic bacterium Colwellia sp. NJ341 at near-freezing temperature

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this