Expression of chitinase gene chit1 from Beauveria bassiana

Chitinase gene chit1 was cloned by the polymerase chain reaction (PCR) and sequenced from Beauveria bassiana. The chitinase gene chit1 was ligated into the yeast expression vector pYES2. The expression vector plasmid was transformed into Saccharomyces cerevisiae H158. Gene expression took place upon induction with 2% galactose. The measurement of enzyme activity shows that the expression production can be expressed in active forms and secreted to the medium; the enzyme activity approaches the peak of 0.47 U/mL after being cultured for 48 h.