Expression of a novel chitinase gene from Trichoderma harzianum in Saccharomyces cerevisiae

Caihong Huang; Qian Yang; Jinzhu Song; Yingqi Song

doi:10.1109/ICBBE.2007.76

Expression of a novel chitinase gene from Trichoderma harzianum in Saccharomyces cerevisiae

Caihong Huang^*
, Qian Yang
, Jinzhu Song
, Yingqi Song

^*Corresponding author for this work

Faculty of Life Sciences and Medicine

Harbin Institute of Technology

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

1 Scopus citations

Abstract

Full-length cDNA encoding endochitinase Chit37 was obtained successfully by construction of T. harzianum mycelium cDNA library and sequencing and bioinformatic analysis of the random selection clones. The results indicated that the open reading frame of Chit37 was 1, 032 bp encoding a protein of 343 amino acids and the deduced molecular weight was 36.6 kDa. The gene was amplified by PCR and then integrated into the genomic DNA of pYES2 by insertion into a single site for recombination, yielding the recombinant pYES2/Chit37 which was used to transform S. cerevisiae H158. Chitinase expressed by pYES2/ Chit37 was induced by galactose (maximal activity 0.75 units ml^-1). The enzyme activity reached the peak expression after cultured for 96 h. The optimal temperature of Chit37 is 40°C and the optimal pH is 5.0.

Original language	English
Title of host publication	2007 1st International Conference on Bioinformatics and Biomedical Engineering, ICBBE
Pages	283-285
Number of pages	3
DOIs	https://doi.org/10.1109/ICBBE.2007.76
State	Published - 2007
Event	2007 1st International Conference on Bioinformatics and Biomedical Engineering, ICBBE - Wuhan, China Duration: 6 Jul 2007 → 8 Jul 2007

Publication series

Name	2007 1st International Conference on Bioinformatics and Biomedical Engineering, ICBBE

Conference

Conference	2007 1st International Conference on Bioinformatics and Biomedical Engineering, ICBBE
Country/Territory	China
City	Wuhan
Period	6/07/07 → 8/07/07

Keywords

Chitinase
Expression
T. Harzianum

Access to Document

10.1109/ICBBE.2007.76

Cite this

Huang, C., Yang, Q., Song, J., & Song, Y. (2007). Expression of a novel chitinase gene from Trichoderma harzianum in Saccharomyces cerevisiae. In 2007 1st International Conference on Bioinformatics and Biomedical Engineering, ICBBE (pp. 283-285). Article 4272560 (2007 1st International Conference on Bioinformatics and Biomedical Engineering, ICBBE). https://doi.org/10.1109/ICBBE.2007.76

@inproceedings{0223e1d201584c1bb517f7989aa2cff4,

title = "Expression of a novel chitinase gene from Trichoderma harzianum in Saccharomyces cerevisiae",

abstract = "Full-length cDNA encoding endochitinase Chit37 was obtained successfully by construction of T. harzianum mycelium cDNA library and sequencing and bioinformatic analysis of the random selection clones. The results indicated that the open reading frame of Chit37 was 1, 032 bp encoding a protein of 343 amino acids and the deduced molecular weight was 36.6 kDa. The gene was amplified by PCR and then integrated into the genomic DNA of pYES2 by insertion into a single site for recombination, yielding the recombinant pYES2/Chit37 which was used to transform S. cerevisiae H158. Chitinase expressed by pYES2/ Chit37 was induced by galactose (maximal activity 0.75 units ml-1). The enzyme activity reached the peak expression after cultured for 96 h. The optimal temperature of Chit37 is 40°C and the optimal pH is 5.0.",

keywords = "Chitinase, Expression, T. Harzianum",

author = "Caihong Huang and Qian Yang and Jinzhu Song and Yingqi Song",

year = "2007",

doi = "10.1109/ICBBE.2007.76",

language = "英语",

isbn = "1424411203",

series = "2007 1st International Conference on Bioinformatics and Biomedical Engineering, ICBBE",

pages = "283--285",

booktitle = "2007 1st International Conference on Bioinformatics and Biomedical Engineering, ICBBE",

note = "2007 1st International Conference on Bioinformatics and Biomedical Engineering, ICBBE ; Conference date: 06-07-2007 Through 08-07-2007",

}

Huang, C, Yang, Q, Song, J & Song, Y 2007, Expression of a novel chitinase gene from Trichoderma harzianum in Saccharomyces cerevisiae. in 2007 1st International Conference on Bioinformatics and Biomedical Engineering, ICBBE., 4272560, 2007 1st International Conference on Bioinformatics and Biomedical Engineering, ICBBE, pp. 283-285, 2007 1st International Conference on Bioinformatics and Biomedical Engineering, ICBBE, Wuhan, China, 6/07/07. https://doi.org/10.1109/ICBBE.2007.76

Expression of a novel chitinase gene from Trichoderma harzianum in Saccharomyces cerevisiae. / Huang, Caihong; Yang, Qian; Song, Jinzhu et al.
2007 1st International Conference on Bioinformatics and Biomedical Engineering, ICBBE. 2007. p. 283-285 4272560 (2007 1st International Conference on Bioinformatics and Biomedical Engineering, ICBBE).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Expression of a novel chitinase gene from Trichoderma harzianum in Saccharomyces cerevisiae

AU - Huang, Caihong

AU - Yang, Qian

AU - Song, Jinzhu

AU - Song, Yingqi

PY - 2007

Y1 - 2007

N2 - Full-length cDNA encoding endochitinase Chit37 was obtained successfully by construction of T. harzianum mycelium cDNA library and sequencing and bioinformatic analysis of the random selection clones. The results indicated that the open reading frame of Chit37 was 1, 032 bp encoding a protein of 343 amino acids and the deduced molecular weight was 36.6 kDa. The gene was amplified by PCR and then integrated into the genomic DNA of pYES2 by insertion into a single site for recombination, yielding the recombinant pYES2/Chit37 which was used to transform S. cerevisiae H158. Chitinase expressed by pYES2/ Chit37 was induced by galactose (maximal activity 0.75 units ml-1). The enzyme activity reached the peak expression after cultured for 96 h. The optimal temperature of Chit37 is 40°C and the optimal pH is 5.0.

AB - Full-length cDNA encoding endochitinase Chit37 was obtained successfully by construction of T. harzianum mycelium cDNA library and sequencing and bioinformatic analysis of the random selection clones. The results indicated that the open reading frame of Chit37 was 1, 032 bp encoding a protein of 343 amino acids and the deduced molecular weight was 36.6 kDa. The gene was amplified by PCR and then integrated into the genomic DNA of pYES2 by insertion into a single site for recombination, yielding the recombinant pYES2/Chit37 which was used to transform S. cerevisiae H158. Chitinase expressed by pYES2/ Chit37 was induced by galactose (maximal activity 0.75 units ml-1). The enzyme activity reached the peak expression after cultured for 96 h. The optimal temperature of Chit37 is 40°C and the optimal pH is 5.0.

KW - Chitinase

KW - Expression

KW - T. Harzianum

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U2 - 10.1109/ICBBE.2007.76

DO - 10.1109/ICBBE.2007.76

M3 - 会议稿件

AN - SCOPUS:35348915419

SN - 1424411203

SN - 9781424411207

T3 - 2007 1st International Conference on Bioinformatics and Biomedical Engineering, ICBBE

SP - 283

EP - 285

BT - 2007 1st International Conference on Bioinformatics and Biomedical Engineering, ICBBE

T2 - 2007 1st International Conference on Bioinformatics and Biomedical Engineering, ICBBE

Y2 - 6 July 2007 through 8 July 2007

ER -

Expression of a novel chitinase gene from Trichoderma harzianum in Saccharomyces cerevisiae

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