Expansion Microscopy Imaging Technique and Its Application

Kai CHEN; Ying Chun ZHANG; Guan Fang ZHAO

doi:10.1016/S1872-2040(19)61156-4

Expansion Microscopy Imaging Technique and Its Application

Kai CHEN
, Ying Chun ZHANG^*
, Guan Fang ZHAO

^*Corresponding author for this work

School of Chemistry and Chemical Engineering, Harbin Institute of Technology
CAS - Changchun Institute of Applied Chemistry

Research output: Contribution to journal › Review article › peer-review

1 Scopus citations

Abstract

Due to the limitations of optical diffraction, traditional optical microscopes have insufficient resolution to observe subcellular microstructures. Super-resolution microscopy, awarded the Nobel Prize in Chemistry in 2014, has been used extensively in single-molecule imaging of microstructures. As a new super-resolution microscopy, expansion microscopy (ExM) has the advantages of short imaging time and can mark dense biological macromolecules. This paper describes the imaging principles and characteristics of ExM, and reviews its applications in subcellular structures, neurobiology and other fields.

Original language	English
Pages (from-to)	643-651
Number of pages	9
Journal	Chinese Journal of Analytical Chemistry
Volume	47
Issue number	5
DOIs	https://doi.org/10.1016/S1872-2040(19)61156-4
State	Published - May 2019
Externally published	Yes

Keywords

Expansion microscopy
Gel
Review
Super-resolution imaging

Access to Document

10.1016/S1872-2040(19)61156-4

Cite this

@article{5ea3884a6c624c4b97eb2a7521291dd4,

title = "Expansion Microscopy Imaging Technique and Its Application",

abstract = "Due to the limitations of optical diffraction, traditional optical microscopes have insufficient resolution to observe subcellular microstructures. Super-resolution microscopy, awarded the Nobel Prize in Chemistry in 2014, has been used extensively in single-molecule imaging of microstructures. As a new super-resolution microscopy, expansion microscopy (ExM) has the advantages of short imaging time and can mark dense biological macromolecules. This paper describes the imaging principles and characteristics of ExM, and reviews its applications in subcellular structures, neurobiology and other fields.",

keywords = "Expansion microscopy, Gel, Review, Super-resolution imaging",

author = "Kai CHEN and ZHANG, \{Ying Chun\} and ZHAO, \{Guan Fang\}",

note = "Publisher Copyright: {\textcopyright} 2019 Changchun Institute of Applied Chemistry, Chinese Academy of Sciences",

year = "2019",

month = may,

doi = "10.1016/S1872-2040(19)61156-4",

language = "英语",

volume = "47",

pages = "643--651",

journal = "Chinese Journal of Analytical Chemistry",

issn = "0253-3820",

publisher = "Chinese Academy of Sciences",

number = "5",

}

TY - JOUR

T1 - Expansion Microscopy Imaging Technique and Its Application

AU - CHEN, Kai

AU - ZHANG, Ying Chun

AU - ZHAO, Guan Fang

PY - 2019/5

Y1 - 2019/5

N2 - Due to the limitations of optical diffraction, traditional optical microscopes have insufficient resolution to observe subcellular microstructures. Super-resolution microscopy, awarded the Nobel Prize in Chemistry in 2014, has been used extensively in single-molecule imaging of microstructures. As a new super-resolution microscopy, expansion microscopy (ExM) has the advantages of short imaging time and can mark dense biological macromolecules. This paper describes the imaging principles and characteristics of ExM, and reviews its applications in subcellular structures, neurobiology and other fields.

AB - Due to the limitations of optical diffraction, traditional optical microscopes have insufficient resolution to observe subcellular microstructures. Super-resolution microscopy, awarded the Nobel Prize in Chemistry in 2014, has been used extensively in single-molecule imaging of microstructures. As a new super-resolution microscopy, expansion microscopy (ExM) has the advantages of short imaging time and can mark dense biological macromolecules. This paper describes the imaging principles and characteristics of ExM, and reviews its applications in subcellular structures, neurobiology and other fields.

KW - Expansion microscopy

KW - Gel

KW - Review

KW - Super-resolution imaging

UR - https://www.scopus.com/pages/publications/85065471372

U2 - 10.1016/S1872-2040(19)61156-4

DO - 10.1016/S1872-2040(19)61156-4

M3 - 文献综述

AN - SCOPUS:85065471372

SN - 0253-3820

VL - 47

SP - 643

EP - 651

JO - Chinese Journal of Analytical Chemistry

JF - Chinese Journal of Analytical Chemistry

IS - 5

ER -

Expansion Microscopy Imaging Technique and Its Application

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this