Establishment of chromium detecting kit and its application in sea water and seafood

Cuijuan Shi; Xiujun Gao; Qiuju Xie; Guangfeng Kan

doi:10.1051/e3sconf/202125203069

Establishment of chromium detecting kit and its application in sea water and seafood

Cuijuan Shi
, Xiujun Gao
, Qiuju Xie
, Guangfeng Kan^*

^*Corresponding author for this work

School of Marine Science and Technology, Harbin Institute of Technology Weihai

Research output: Contribution to journal › Conference article › peer-review

Abstract

Chromium is one of main pollution in sea water, and the establishment of detecting kit is the research hot topic. In this study, immunized mice were obtained by immunogen Cr3+-EDTA-BSA, and hybridoma strains that secrete Cr3+-EDTA monoclonal antibody were selected. Cr3+-EDTA mAb were prepared with in vivo method. Then, Cr-kit for measurement of chromium was developed, and its application was executed in sea water and seafood. Two hybridoma strains of A321 and A424 were screened. The Cr-kit standard curve of the linear range was 1.0 - 512 µg/L, and limit of detection was 1.0 µg/L. The average recovery rates Cr spiked in samples of sea water, clam and shrimp were 102.62%, 94.69% and 96.29%, which were accordance with the results of ICP-AES. Totally, Cr-kit was successfully developed and behaved broad application potential in sea water and seafood.

Original language	English
Article number	03069
Journal	E3S Web of Conferences
Volume	252
DOIs	https://doi.org/10.1051/e3sconf/202125203069
State	Published - 23 Apr 2021
Externally published	Yes
Event	2021 International Conference on Power Grid System and Green Energy, PGSGE 2021 - Wuhan, China Duration: 26 Mar 2021 → 28 Mar 2021

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@article{c6a8c178ef5b497d8ff42449186ed102,

title = "Establishment of chromium detecting kit and its application in sea water and seafood",

abstract = "Chromium is one of main pollution in sea water, and the establishment of detecting kit is the research hot topic. In this study, immunized mice were obtained by immunogen Cr3+-EDTA-BSA, and hybridoma strains that secrete Cr3+-EDTA monoclonal antibody were selected. Cr3+-EDTA mAb were prepared with in vivo method. Then, Cr-kit for measurement of chromium was developed, and its application was executed in sea water and seafood. Two hybridoma strains of A321 and A424 were screened. The Cr-kit standard curve of the linear range was 1.0 - 512 µg/L, and limit of detection was 1.0 µg/L. The average recovery rates Cr spiked in samples of sea water, clam and shrimp were 102.62\%, 94.69\% and 96.29\%, which were accordance with the results of ICP-AES. Totally, Cr-kit was successfully developed and behaved broad application potential in sea water and seafood.",

author = "Cuijuan Shi and Xiujun Gao and Qiuju Xie and Guangfeng Kan",

note = "Publisher Copyright: {\textcopyright} The Authors, published by EDP Sciences, 2021.; 2021 International Conference on Power Grid System and Green Energy, PGSGE 2021 ; Conference date: 26-03-2021 Through 28-03-2021",

year = "2021",

month = apr,

day = "23",

doi = "10.1051/e3sconf/202125203069",

language = "英语",

volume = "252",

journal = "E3S Web of Conferences",

issn = "2267-1242",

publisher = "EDP Sciences",

}

TY - JOUR

T1 - Establishment of chromium detecting kit and its application in sea water and seafood

AU - Shi, Cuijuan

AU - Gao, Xiujun

AU - Xie, Qiuju

AU - Kan, Guangfeng

N1 - Publisher Copyright: © The Authors, published by EDP Sciences, 2021.

PY - 2021/4/23

Y1 - 2021/4/23

N2 - Chromium is one of main pollution in sea water, and the establishment of detecting kit is the research hot topic. In this study, immunized mice were obtained by immunogen Cr3+-EDTA-BSA, and hybridoma strains that secrete Cr3+-EDTA monoclonal antibody were selected. Cr3+-EDTA mAb were prepared with in vivo method. Then, Cr-kit for measurement of chromium was developed, and its application was executed in sea water and seafood. Two hybridoma strains of A321 and A424 were screened. The Cr-kit standard curve of the linear range was 1.0 - 512 µg/L, and limit of detection was 1.0 µg/L. The average recovery rates Cr spiked in samples of sea water, clam and shrimp were 102.62%, 94.69% and 96.29%, which were accordance with the results of ICP-AES. Totally, Cr-kit was successfully developed and behaved broad application potential in sea water and seafood.

AB - Chromium is one of main pollution in sea water, and the establishment of detecting kit is the research hot topic. In this study, immunized mice were obtained by immunogen Cr3+-EDTA-BSA, and hybridoma strains that secrete Cr3+-EDTA monoclonal antibody were selected. Cr3+-EDTA mAb were prepared with in vivo method. Then, Cr-kit for measurement of chromium was developed, and its application was executed in sea water and seafood. Two hybridoma strains of A321 and A424 were screened. The Cr-kit standard curve of the linear range was 1.0 - 512 µg/L, and limit of detection was 1.0 µg/L. The average recovery rates Cr spiked in samples of sea water, clam and shrimp were 102.62%, 94.69% and 96.29%, which were accordance with the results of ICP-AES. Totally, Cr-kit was successfully developed and behaved broad application potential in sea water and seafood.

UR - https://www.scopus.com/pages/publications/85105368991

U2 - 10.1051/e3sconf/202125203069

DO - 10.1051/e3sconf/202125203069

M3 - 会议文章

AN - SCOPUS:85105368991

SN - 2267-1242

VL - 252

JO - E3S Web of Conferences

JF - E3S Web of Conferences

M1 - 03069

T2 - 2021 International Conference on Power Grid System and Green Energy, PGSGE 2021

Y2 - 26 March 2021 through 28 March 2021

ER -

Establishment of chromium detecting kit and its application in sea water and seafood

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