Enhanced performance and microbial interactions of shallow wetland bed coupling with functional biocathode microbial electrochemical system (MES)

It is essential to remediate the polluted urban river, which endangers the aquatic creatures and affected human body's senses. The treatment wetland combined with microbial electrochemical system (MES) used for the remediation is becoming a new research focus due to its ideal pollutants removal efficiency and small footprint. Here this paper provided a kind of novel shallow wetland bed coupling with close-circuit microbial electrochemical system (WB-CMES) to remove pollutants in surface water. In contrast to the shallow wetland bed coupling with open-circuit MES (WB-OMES) and the shallow wetland bed without MES (WB), the enhancing effects and pollutants removal pathway were evaluated. After 62-day operation, average TN removal efficiency in WB-CMES was 87.7%, which was 19.7% and 13.8% higher than that of WB-OMES and WB respectively. The rate coefficient k of NO₃⁻–N degradation in WB-CMES was 1.6 and 1.8 times higher than that in WB-OMES and WB. The results of chlorophyll, protein and superoxide dismutase (SOD) in WB-CMES were 27.3%, 44.3% and 12.9% higher than those in WB. The microbial community structure analysis indicated that electroactive bacteria on anode like Desulfobulbus could oxidize organics and generate electrons to compensate cathode, meanwhile, cathode could enrich more species of functional bacteria like Rhodobacter, Pirellula, Hyphomicrobium, Thauera, which had a synergistic effect on oxygen reduction, nitrogen removal and plant growth. The results indicated that oxygen produced by submerged plants could be utilized by the oxygen-reducing functional biocathode of MES and the proper aerobic and anoxic environment might enhance nitrate removal mainly through simultaneous nitrification and denitrification (SND), aerobic denitrification and anammox. This research provided a novel technology with advantages of simple operation, flexible configuration, easy scale-up and low cost for application in remediation of highly polluted surface water.