Enhanced amplification of GC-rich DNA with two organic reagents

Zhizhou Zhang; Xia Yang; Liangyu Meng; Fang Liu; Cenchao Shen; Wenjuan Yang

doi:10.2144/000113203

Enhanced amplification of GC-rich DNA with two organic reagents

Zhizhou Zhang^*
, Xia Yang
, Liangyu Meng
, Fang Liu
, Cenchao Shen
, Wenjuan Yang

^*Corresponding author for this work

Harbin Institute of Technology Weihai
Tianjin University of Science & Technology

Research output: Contribution to journal › Article › peer-review

51 Scopus citations

Abstract

Amplification of GC-rich DNA sequences is still a difficult task worldwide. Two frequently seen and inexpensive reagents - ethylene glycol and 1,2-propanediol - were found to be more effective than betaine in the amplification of 104 randomly selected GC-rich human DNA sequences with GC contents of 60-80% and lengths of 700-800 bp.

Original language	English
Pages (from-to)	775-779
Number of pages	5
Journal	BioTechniques
Volume	47
Issue number	3
DOIs	https://doi.org/10.2144/000113203
State	Published - Sep 2009
Externally published	Yes

Keywords

1,2-Propanediol
Ethylene glycol
GC-rich DNA
PCR

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10.2144/000113203

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title = "Enhanced amplification of GC-rich DNA with two organic reagents",

abstract = "Amplification of GC-rich DNA sequences is still a difficult task worldwide. Two frequently seen and inexpensive reagents - ethylene glycol and 1,2-propanediol - were found to be more effective than betaine in the amplification of 104 randomly selected GC-rich human DNA sequences with GC contents of 60-80\% and lengths of 700-800 bp.",

keywords = "1,2-Propanediol, Ethylene glycol, GC-rich DNA, PCR",

author = "Zhizhou Zhang and Xia Yang and Liangyu Meng and Fang Liu and Cenchao Shen and Wenjuan Yang",

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doi = "10.2144/000113203",

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T1 - Enhanced amplification of GC-rich DNA with two organic reagents

AU - Zhang, Zhizhou

AU - Yang, Xia

AU - Meng, Liangyu

AU - Liu, Fang

AU - Shen, Cenchao

AU - Yang, Wenjuan

PY - 2009/9

Y1 - 2009/9

N2 - Amplification of GC-rich DNA sequences is still a difficult task worldwide. Two frequently seen and inexpensive reagents - ethylene glycol and 1,2-propanediol - were found to be more effective than betaine in the amplification of 104 randomly selected GC-rich human DNA sequences with GC contents of 60-80% and lengths of 700-800 bp.

AB - Amplification of GC-rich DNA sequences is still a difficult task worldwide. Two frequently seen and inexpensive reagents - ethylene glycol and 1,2-propanediol - were found to be more effective than betaine in the amplification of 104 randomly selected GC-rich human DNA sequences with GC contents of 60-80% and lengths of 700-800 bp.

KW - 1,2-Propanediol

KW - Ethylene glycol

KW - GC-rich DNA

KW - PCR

UR - https://www.scopus.com/pages/publications/77954069646

U2 - 10.2144/000113203

DO - 10.2144/000113203

M3 - 文章

C2 - 19852763

AN - SCOPUS:77954069646

SN - 0736-6205

VL - 47

SP - 775

EP - 779

JO - BioTechniques

JF - BioTechniques

IS - 3

ER -

Enhanced amplification of GC-rich DNA with two organic reagents

Abstract

Keywords

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