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Effects of RGD peptide grafting to titanium dental implants on the adhesion of human gingival fibroblasts and epithelial cells

  • Bao Hong Zhao
  • , Wei Ming Tian
  • , Hai Lan Feng
  • , In Seop Lee*
  • , Fu Zhai Cui
  • *Corresponding author for this work
  • China Medical University
  • Peking University
  • Yonsei University
  • Tsinghua University

Research output: Contribution to journalArticlepeer-review

Abstract

The aim of this study is to test the adhesion of human gingival fibroblasts (HGFs) and epithelial cells (HGEs) to titanium surface by RGD grafting for dental implant, and to obtain an effective perimucosal seal of soft tissue to the implant. RGD peptides were covalently attached to the titanium surface by CDI activation method to improve the adhesion of gingival cells to the titanium dental implants. HGFs and HGEs were inoculated on commercially pure (CP) titanium and RGD-grafted titanium. The number of attached and proliferated cells was quantified by morphometric analysis using immunofluorescence. The results showed that the bioactive peptide RGD was grafted on CP titanium by CDI activation method. The RGD grafting exhibited that the number of attached and proliferated fibroblasts and epithelial cells were higher than that on CP titanium. The RGD-grafting is advantageous to gingival cells adherence to the surface of titanium implant.

Original languageEnglish
Pages (from-to)407-410
Number of pages4
JournalCurrent Applied Physics
Volume5
Issue number5
DOIs
StatePublished - Jul 2005
Externally publishedYes

Keywords

  • Cell adhesion
  • Dental implant
  • Epithelial cells
  • Fibroblasts
  • RGD

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