Effect of incubation conditions and possible intestinal nutrients on cis-9, trans-11 conjugated linoleic acid production by Lactobacillus acidophilus F0221

Jingyan Li; Lanwei Zhang; Xue Han; Huaxi Yi; Chunfeng Guo; Yingchun Zhang; Ming Du; Xue Luo; Yanhe Zhang; Yujuan Shan

doi:10.1016/j.idairyj.2012.10.013

Effect of incubation conditions and possible intestinal nutrients on cis-9, trans-11 conjugated linoleic acid production by Lactobacillus acidophilus F0221

Jingyan Li
, Lanwei Zhang^*
, Xue Han
, Huaxi Yi
, Chunfeng Guo
, Yingchun Zhang
, Ming Du
, Xue Luo
, Yanhe Zhang
, Yujuan Shan

^*Corresponding author for this work

Harbin Institute of Technology
Northwest Agriculture and Forestry University
Chinese Academy of Agricultural Sciences

Research output: Contribution to journal › Article › peer-review

30 Scopus citations

Abstract

The purpose of this study was to optimise the incubation conditions for cis-9, trans-11 conjugated linoleic acid (c9, t11 CLA) production by Lactobacillus acidophilus F0221 and evaluate the effect of possible intestinal nutrients on c9, t11 CLA production. Growth in Mann Rogosa Sharp broth supplemented with cysteine and containing 0.5 g L^-1 linoleic acid at pH 6.5 with anaerobic incubation at 37 °C for 40 h were found to be the optimal conditions for CLA production. Galactosaccharide, arabinogalactan, galactose and glucose had a higher promoting effect on CLA production (96.19-123.89 μg mL^-1) than other carbohydrates. Acetic acid had a higher promoting effect on CLA production (130.98 μg mL^-1) than other short chain fatty acids. These results provide detailed parameters for the production of c9, t11 CLA by L. acidophilus F0221 and are valuable for further understanding of c9, t11 CLA formation in the human intestine by this strain.

Original language	English
Pages (from-to)	93-98
Number of pages	6
Journal	International Dairy Journal
Volume	29
Issue number	2
DOIs	https://doi.org/10.1016/j.idairyj.2012.10.013
State	Published - Apr 2013

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title = "Effect of incubation conditions and possible intestinal nutrients on cis-9, trans-11 conjugated linoleic acid production by Lactobacillus acidophilus F0221",

abstract = "The purpose of this study was to optimise the incubation conditions for cis-9, trans-11 conjugated linoleic acid (c9, t11 CLA) production by Lactobacillus acidophilus F0221 and evaluate the effect of possible intestinal nutrients on c9, t11 CLA production. Growth in Mann Rogosa Sharp broth supplemented with cysteine and containing 0.5 g L-1 linoleic acid at pH 6.5 with anaerobic incubation at 37 °C for 40 h were found to be the optimal conditions for CLA production. Galactosaccharide, arabinogalactan, galactose and glucose had a higher promoting effect on CLA production (96.19-123.89 μg mL-1) than other carbohydrates. Acetic acid had a higher promoting effect on CLA production (130.98 μg mL-1) than other short chain fatty acids. These results provide detailed parameters for the production of c9, t11 CLA by L. acidophilus F0221 and are valuable for further understanding of c9, t11 CLA formation in the human intestine by this strain.",

author = "Jingyan Li and Lanwei Zhang and Xue Han and Huaxi Yi and Chunfeng Guo and Yingchun Zhang and Ming Du and Xue Luo and Yanhe Zhang and Yujuan Shan",

year = "2013",

month = apr,

doi = "10.1016/j.idairyj.2012.10.013",

language = "英语",

volume = "29",

pages = "93--98",

journal = "International Dairy Journal",

issn = "0958-6946",

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T1 - Effect of incubation conditions and possible intestinal nutrients on cis-9, trans-11 conjugated linoleic acid production by Lactobacillus acidophilus F0221

AU - Li, Jingyan

AU - Zhang, Lanwei

AU - Han, Xue

AU - Yi, Huaxi

AU - Guo, Chunfeng

AU - Zhang, Yingchun

AU - Du, Ming

AU - Luo, Xue

AU - Zhang, Yanhe

AU - Shan, Yujuan

PY - 2013/4

Y1 - 2013/4

N2 - The purpose of this study was to optimise the incubation conditions for cis-9, trans-11 conjugated linoleic acid (c9, t11 CLA) production by Lactobacillus acidophilus F0221 and evaluate the effect of possible intestinal nutrients on c9, t11 CLA production. Growth in Mann Rogosa Sharp broth supplemented with cysteine and containing 0.5 g L-1 linoleic acid at pH 6.5 with anaerobic incubation at 37 °C for 40 h were found to be the optimal conditions for CLA production. Galactosaccharide, arabinogalactan, galactose and glucose had a higher promoting effect on CLA production (96.19-123.89 μg mL-1) than other carbohydrates. Acetic acid had a higher promoting effect on CLA production (130.98 μg mL-1) than other short chain fatty acids. These results provide detailed parameters for the production of c9, t11 CLA by L. acidophilus F0221 and are valuable for further understanding of c9, t11 CLA formation in the human intestine by this strain.

AB - The purpose of this study was to optimise the incubation conditions for cis-9, trans-11 conjugated linoleic acid (c9, t11 CLA) production by Lactobacillus acidophilus F0221 and evaluate the effect of possible intestinal nutrients on c9, t11 CLA production. Growth in Mann Rogosa Sharp broth supplemented with cysteine and containing 0.5 g L-1 linoleic acid at pH 6.5 with anaerobic incubation at 37 °C for 40 h were found to be the optimal conditions for CLA production. Galactosaccharide, arabinogalactan, galactose and glucose had a higher promoting effect on CLA production (96.19-123.89 μg mL-1) than other carbohydrates. Acetic acid had a higher promoting effect on CLA production (130.98 μg mL-1) than other short chain fatty acids. These results provide detailed parameters for the production of c9, t11 CLA by L. acidophilus F0221 and are valuable for further understanding of c9, t11 CLA formation in the human intestine by this strain.

UR - https://www.scopus.com/pages/publications/84872258287

U2 - 10.1016/j.idairyj.2012.10.013

DO - 10.1016/j.idairyj.2012.10.013

M3 - 文章

AN - SCOPUS:84872258287

SN - 0958-6946

VL - 29

SP - 93

EP - 98

JO - International Dairy Journal

JF - International Dairy Journal

IS - 2

ER -

Effect of incubation conditions and possible intestinal nutrients on cis-9, trans-11 conjugated linoleic acid production by Lactobacillus acidophilus F0221

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