Abstract
The hydrolytic reaction of L-α-dipalmitoylphosphatidylcholine (L-DPPC) monolayer catalyzed by phospholipase C (PLC) has been investigated using monomolecular membrane technique and Brewster angle microscopy (BAM) at the air/water interface. The curves of surface pressure as a function of time determined a lag-burst process of L-DPPC monolayer hydrolysis by PLC. The BAM images recorded the changes of domains formed in the coexistence region of liquid-condensed (LC) and liquid-expanded (LE) phases during the monolayer hydrolysis. The changes of domain shape and size and the increase of domain number reflect the decrease of reactant and molecular rearrangement process of the main hydrolysis product, dipalmitoylglycerol (DPG). A new phase was observed after the hydrolysis reaction was completed.
| Original language | English |
|---|---|
| Pages (from-to) | 541-545 |
| Number of pages | 5 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 300 |
| Issue number | 2 |
| DOIs | |
| State | Published - 10 Jan 2003 |
| Externally published | Yes |
Keywords
- Brewster angle microscopy
- Hydrolysis
- Phospholipase C
- Phospholipid monolayer
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