DNAzyme-powered nucleic acid release from solid supports

Ting Cao; Yongcheng Wang; Ye Tao; Lexiang Zhang; Ying Lin Zhou; Xin Xiang Zhang; John A. Heyman; David A. Weitz

doi:10.1039/c9cc07790a

DNAzyme-powered nucleic acid release from solid supports

Ting Cao
, Yongcheng Wang
, Ye Tao
, Lexiang Zhang
, Ying Lin Zhou^*
, Xin Xiang Zhang
, John A. Heyman
, David A. Weitz

^*Corresponding author for this work

Harvard University
Wyss Institute for Biologically Inspired Engineering
Ministry of Education of the People's Republic of China

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Here, we demonstrate use of a Mg²⁺-dependent, site-specific DNA enzyme (DNAzyme) to cleave oligos from polyacrylamide gel beads, which is suitable for use in drop-based assays. We show that cleavage efficiency is improved by use of a tandem-repeat cleavage site. We further demonstrate that DNAzyme-released oligos function as primers in reverse transcription of cell-released mRNA.

Original language	English
Pages (from-to)	647-650
Number of pages	4
Journal	Chemical Communications
Volume	56
Issue number	4
DOIs	https://doi.org/10.1039/c9cc07790a
State	Published - 2020
Externally published	Yes

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10.1039/c9cc07790a

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title = "DNAzyme-powered nucleic acid release from solid supports",

abstract = "Here, we demonstrate use of a Mg2+-dependent, site-specific DNA enzyme (DNAzyme) to cleave oligos from polyacrylamide gel beads, which is suitable for use in drop-based assays. We show that cleavage efficiency is improved by use of a tandem-repeat cleavage site. We further demonstrate that DNAzyme-released oligos function as primers in reverse transcription of cell-released mRNA.",

author = "Ting Cao and Yongcheng Wang and Ye Tao and Lexiang Zhang and Zhou, \{Ying Lin\} and Zhang, \{Xin Xiang\} and Heyman, \{John A.\} and Weitz, \{David A.\}",

note = "Publisher Copyright: {\textcopyright} 2020 The Royal Society of Chemistry.",

year = "2020",

doi = "10.1039/c9cc07790a",

language = "英语",

volume = "56",

pages = "647--650",

journal = "Chemical Communications",

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TY - JOUR

T1 - DNAzyme-powered nucleic acid release from solid supports

AU - Cao, Ting

AU - Wang, Yongcheng

AU - Tao, Ye

AU - Zhang, Lexiang

AU - Zhou, Ying Lin

AU - Zhang, Xin Xiang

AU - Heyman, John A.

AU - Weitz, David A.

PY - 2020

Y1 - 2020

N2 - Here, we demonstrate use of a Mg2+-dependent, site-specific DNA enzyme (DNAzyme) to cleave oligos from polyacrylamide gel beads, which is suitable for use in drop-based assays. We show that cleavage efficiency is improved by use of a tandem-repeat cleavage site. We further demonstrate that DNAzyme-released oligos function as primers in reverse transcription of cell-released mRNA.

AB - Here, we demonstrate use of a Mg2+-dependent, site-specific DNA enzyme (DNAzyme) to cleave oligos from polyacrylamide gel beads, which is suitable for use in drop-based assays. We show that cleavage efficiency is improved by use of a tandem-repeat cleavage site. We further demonstrate that DNAzyme-released oligos function as primers in reverse transcription of cell-released mRNA.

UR - https://www.scopus.com/pages/publications/85077727357

U2 - 10.1039/c9cc07790a

DO - 10.1039/c9cc07790a

M3 - 文章

C2 - 31840153

AN - SCOPUS:85077727357

SN - 1359-7345

VL - 56

SP - 647

EP - 650

JO - Chemical Communications

JF - Chemical Communications

IS - 4

ER -

DNAzyme-powered nucleic acid release from solid supports

Abstract

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