DNA damage and effects on antioxidative enzymes in mouse marrow induced by berberine

Chengbin Xu; Lu Wang; Xiujuan Hui; Xiping Ma; Fang Ma; Quan Zhao; Xin Bi

doi:10.2495/HHME130851

DNA damage and effects on antioxidative enzymes in mouse marrow induced by berberine

Chengbin Xu
, Lu Wang
, Xiujuan Hui
, Xiping Ma
, Fang Ma
, Quan Zhao
, Xin Bi

School of Environment

Harbin Institute of Technology
Liaoning University

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

1 Scopus citations

Abstract

Berberine, an inflammatory medicine, which is widely used in clinical medicine, is rarely studied about its affection on nontarget organisms. To evaluate the ecotoxicity of berberine in mouse marrow cells, biochemical responses including DNA damage and changes in the activities of antioxidative enzymes induced by berberine were investigated in mouse marrow cells. 7.5, 15, 30, 60, and 120 mg/kg berberine was added to mouse marrow cells, respectively. Compared to the controls, the damage, tail DNA content (%), tail length and tail moment went up, respectively, in dose-effect manner following the rising berberine. As the content of berberine increased, the activities of superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD) were gradually decreased and the concentration of malondialdehyde (MDA) was gradually increased. Compared to the controls, the change tendency showed significant differences (p < 0.05 or p < 0.01). In conclusion, berberine induces oxidative stress and DNA damage to marrow cells and has genotoxicity.

Original language	English
Title of host publication	Human Health and Medical Engineering
Editors	Zhenyu Du, Maozhu Jin
Publisher	WITPress
Pages	479-486
Number of pages	8
ISBN (Electronic)	9781845648923
ISBN (Print)	9781845648923
DOIs	https://doi.org/10.2495/HHME130851
State	Published - 2014
Event	2013 International Conference on Human Health and Medical Engineering, HHME 2013 - Wuhan, China Duration: 7 Dec 2013 → 8 Dec 2013

Publication series

Name	WIT Transactions on Biomedicine and Health
Volume	18
ISSN (Print)	1743-3525

Conference

Conference	2013 International Conference on Human Health and Medical Engineering, HHME 2013
Country/Territory	China
City	Wuhan
Period	7/12/13 → 8/12/13

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Berberine
Comet assay
DNA damage
Genotoxicity
Marrow cells

Access to Document

10.2495/HHME130851

Cite this

@inproceedings{27b63c226fc34c0b8645682a68da0ec3,

title = "DNA damage and effects on antioxidative enzymes in mouse marrow induced by berberine",

abstract = "Berberine, an inflammatory medicine, which is widely used in clinical medicine, is rarely studied about its affection on nontarget organisms. To evaluate the ecotoxicity of berberine in mouse marrow cells, biochemical responses including DNA damage and changes in the activities of antioxidative enzymes induced by berberine were investigated in mouse marrow cells. 7.5, 15, 30, 60, and 120 mg/kg berberine was added to mouse marrow cells, respectively. Compared to the controls, the damage, tail DNA content (\%), tail length and tail moment went up, respectively, in dose-effect manner following the rising berberine. As the content of berberine increased, the activities of superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD) were gradually decreased and the concentration of malondialdehyde (MDA) was gradually increased. Compared to the controls, the change tendency showed significant differences (p < 0.05 or p < 0.01). In conclusion, berberine induces oxidative stress and DNA damage to marrow cells and has genotoxicity.",

keywords = "Berberine, Comet assay, DNA damage, Genotoxicity, Marrow cells",

author = "Chengbin Xu and Lu Wang and Xiujuan Hui and Xiping Ma and Fang Ma and Quan Zhao and Xin Bi",

year = "2014",

doi = "10.2495/HHME130851",

language = "英语",

isbn = "9781845648923",

series = "WIT Transactions on Biomedicine and Health",

publisher = "WITPress",

pages = "479--486",

editor = "Zhenyu Du and Maozhu Jin",

booktitle = "Human Health and Medical Engineering",

address = "英国",

note = "2013 International Conference on Human Health and Medical Engineering, HHME 2013 ; Conference date: 07-12-2013 Through 08-12-2013",

}

Xu, C, Wang, L, Hui, X, Ma, X, Ma, F, Zhao, Q & Bi, X 2014, DNA damage and effects on antioxidative enzymes in mouse marrow induced by berberine. in Z Du & M Jin (eds), Human Health and Medical Engineering. WIT Transactions on Biomedicine and Health, vol. 18, WITPress, pp. 479-486, 2013 International Conference on Human Health and Medical Engineering, HHME 2013, Wuhan, China, 7/12/13. https://doi.org/10.2495/HHME130851

DNA damage and effects on antioxidative enzymes in mouse marrow induced by berberine. / Xu, Chengbin; Wang, Lu; Hui, Xiujuan et al.
Human Health and Medical Engineering. ed. / Zhenyu Du; Maozhu Jin. WITPress, 2014. p. 479-486 (WIT Transactions on Biomedicine and Health; Vol. 18).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - DNA damage and effects on antioxidative enzymes in mouse marrow induced by berberine

AU - Xu, Chengbin

AU - Wang, Lu

AU - Hui, Xiujuan

AU - Ma, Xiping

AU - Ma, Fang

AU - Zhao, Quan

AU - Bi, Xin

PY - 2014

Y1 - 2014

N2 - Berberine, an inflammatory medicine, which is widely used in clinical medicine, is rarely studied about its affection on nontarget organisms. To evaluate the ecotoxicity of berberine in mouse marrow cells, biochemical responses including DNA damage and changes in the activities of antioxidative enzymes induced by berberine were investigated in mouse marrow cells. 7.5, 15, 30, 60, and 120 mg/kg berberine was added to mouse marrow cells, respectively. Compared to the controls, the damage, tail DNA content (%), tail length and tail moment went up, respectively, in dose-effect manner following the rising berberine. As the content of berberine increased, the activities of superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD) were gradually decreased and the concentration of malondialdehyde (MDA) was gradually increased. Compared to the controls, the change tendency showed significant differences (p < 0.05 or p < 0.01). In conclusion, berberine induces oxidative stress and DNA damage to marrow cells and has genotoxicity.

AB - Berberine, an inflammatory medicine, which is widely used in clinical medicine, is rarely studied about its affection on nontarget organisms. To evaluate the ecotoxicity of berberine in mouse marrow cells, biochemical responses including DNA damage and changes in the activities of antioxidative enzymes induced by berberine were investigated in mouse marrow cells. 7.5, 15, 30, 60, and 120 mg/kg berberine was added to mouse marrow cells, respectively. Compared to the controls, the damage, tail DNA content (%), tail length and tail moment went up, respectively, in dose-effect manner following the rising berberine. As the content of berberine increased, the activities of superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD) were gradually decreased and the concentration of malondialdehyde (MDA) was gradually increased. Compared to the controls, the change tendency showed significant differences (p < 0.05 or p < 0.01). In conclusion, berberine induces oxidative stress and DNA damage to marrow cells and has genotoxicity.

KW - Berberine

KW - Comet assay

KW - DNA damage

KW - Genotoxicity

KW - Marrow cells

UR - https://www.scopus.com/pages/publications/84905178432

U2 - 10.2495/HHME130851

DO - 10.2495/HHME130851

M3 - 会议稿件

AN - SCOPUS:84905178432

SN - 9781845648923

T3 - WIT Transactions on Biomedicine and Health

SP - 479

EP - 486

BT - Human Health and Medical Engineering

A2 - Du, Zhenyu

A2 - Jin, Maozhu

PB - WITPress

T2 - 2013 International Conference on Human Health and Medical Engineering, HHME 2013

Y2 - 7 December 2013 through 8 December 2013

ER -

DNA damage and effects on antioxidative enzymes in mouse marrow induced by berberine

Abstract

Publication series

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UN SDGs

Keywords

Access to Document

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