@inproceedings{f485b0d5e90548e4b2529fb405964021,
title = "Digital-WGS: Automated, highly efficient whole-genome sequencing of single cells by digital microfluidics",
abstract = "Current sample preparation technologies for single-cell whole-genome sequencing (WGS) are complex, expensive, and suffer from high amplification bias and errors. This paper describes Digital-WGS, a sample preparation platform that automates parallel nanoliter-volume WGS from efficient single-cell isolation to high-performance whole-genome amplification based on digital microfluidics. The digital control of droplets in a closed hydrophobic interface enables the complete removal of exogenous DNA, sufficient cell lysis and lossless amplicons recovery, achieving the low CV and high coverage at multiple scales. Digital-WGS provides an efficient and robust method for performing single-cell sequencing, which is scalable and universal for any chemistry of single-cell analysis.",
keywords = "Digital microfluidics, Genome, Sequencing, Single cells, Whole genome amplification",
author = "Qingyu Ruan and Weidong Ruan and Xiaoye Lin and Zhi Zhu and Chaoyong Yang",
note = "Publisher Copyright: {\textcopyright} 2020 CBMS-0001; 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2020 ; Conference date: 04-10-2020 Through 09-10-2020",
year = "2020",
language = "英语",
series = "MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences",
publisher = "Chemical and Biological Microsystems Society",
pages = "252--253",
booktitle = "MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences",
}