Detection of adenine-rich ssDNA based on thymine-substituted tetraphenylethene with aggregation-induced emission characteristics

Xiaoding Lou; Chris Wai Tung Leung; Chao Dong; Yuning Hong; Sijie Chen; Engui Zhao; Jacky Wing Yip Lam; Ben Zhong Tang

doi:10.1039/c4ra05765a

Detection of adenine-rich ssDNA based on thymine-substituted tetraphenylethene with aggregation-induced emission characteristics

Xiaoding Lou
, Chris Wai Tung Leung
, Chao Dong
, Yuning Hong
, Sijie Chen
, Engui Zhao
, Jacky Wing Yip Lam
, Ben Zhong Tang^*

^*Corresponding author for this work

Huazhong University of Science and Technology
Hong Kong University of Science and Technology
South China University of Technology

Research output: Contribution to journal › Review article › peer-review

29 Scopus citations

Abstract

In this work, tetraphenylethene (TPE) equipped with thymine, TPE-T, was synthesized and used as a fluorescence biosensor for differentiating ssDNA from dsDNA. TPE-T is a "turn-on" fluorescence probe once it forms hydrogen bonds with single-stranded adenine-containing oligonucleotides with high sensitivity and specificity. The working mechanism of TPE-T and its potential in cell imaging are investigated in detail.

Original language	English
Pages (from-to)	33307-33311
Number of pages	5
Journal	RSC Advances
Volume	4
Issue number	63
DOIs	https://doi.org/10.1039/c4ra05765a
State	Published - 2014
Externally published	Yes

Access to Document

10.1039/c4ra05765a

Cite this

@article{c9cca69122ca46e79e62dbae37e02832,

title = "Detection of adenine-rich ssDNA based on thymine-substituted tetraphenylethene with aggregation-induced emission characteristics",

abstract = "In this work, tetraphenylethene (TPE) equipped with thymine, TPE-T, was synthesized and used as a fluorescence biosensor for differentiating ssDNA from dsDNA. TPE-T is a {"}turn-on{"} fluorescence probe once it forms hydrogen bonds with single-stranded adenine-containing oligonucleotides with high sensitivity and specificity. The working mechanism of TPE-T and its potential in cell imaging are investigated in detail.",

author = "Xiaoding Lou and Leung, \{Chris Wai Tung\} and Chao Dong and Yuning Hong and Sijie Chen and Engui Zhao and Lam, \{Jacky Wing Yip\} and Tang, \{Ben Zhong\}",

year = "2014",

doi = "10.1039/c4ra05765a",

language = "英语",

volume = "4",

pages = "33307--33311",

journal = "RSC Advances",

issn = "2046-2069",

publisher = "Royal Society of Chemistry",

number = "63",

}

TY - JOUR

T1 - Detection of adenine-rich ssDNA based on thymine-substituted tetraphenylethene with aggregation-induced emission characteristics

AU - Lou, Xiaoding

AU - Leung, Chris Wai Tung

AU - Dong, Chao

AU - Hong, Yuning

AU - Chen, Sijie

AU - Zhao, Engui

AU - Lam, Jacky Wing Yip

AU - Tang, Ben Zhong

PY - 2014

Y1 - 2014

N2 - In this work, tetraphenylethene (TPE) equipped with thymine, TPE-T, was synthesized and used as a fluorescence biosensor for differentiating ssDNA from dsDNA. TPE-T is a "turn-on" fluorescence probe once it forms hydrogen bonds with single-stranded adenine-containing oligonucleotides with high sensitivity and specificity. The working mechanism of TPE-T and its potential in cell imaging are investigated in detail.

AB - In this work, tetraphenylethene (TPE) equipped with thymine, TPE-T, was synthesized and used as a fluorescence biosensor for differentiating ssDNA from dsDNA. TPE-T is a "turn-on" fluorescence probe once it forms hydrogen bonds with single-stranded adenine-containing oligonucleotides with high sensitivity and specificity. The working mechanism of TPE-T and its potential in cell imaging are investigated in detail.

UR - https://www.scopus.com/pages/publications/84905717879

U2 - 10.1039/c4ra05765a

DO - 10.1039/c4ra05765a

M3 - 文献综述

AN - SCOPUS:84905717879

SN - 2046-2069

VL - 4

SP - 33307

EP - 33311

JO - RSC Advances

JF - RSC Advances

IS - 63

ER -

Detection of adenine-rich ssDNA based on thymine-substituted tetraphenylethene with aggregation-induced emission characteristics

Abstract

Access to Document

Other files and links

Fingerprint

Cite this