TY - GEN
T1 - Denitrification characteristics, pH and temperature influence of an aerobic denitrifier
AU - Wang, Qiang
AU - Yang, Jixian
AU - Shang, Ran
AU - Zhang, Xiaoqi
AU - Ma, Fang
PY - 2009
Y1 - 2009
N2 - A high efficient aerobic denitrifying bacterium(G2) capable of rapid removal of NO3- and NO2-was isolated from activated sludge. The purpose of this work was to study the characteristics, pH and temperature effects of the isolated bacterium. The morphology of the isolated aerobic denitrifier G2 was a short bacillus, with average size of (0.3-0.6)μm× (1.4-3.0) μm. According to the16S rDNA sequencing analysis, the isolated G2 was identified to be Paracoccus sp. The napA gene was cloned from G2. The gene was subunit of periplasmic nitrate reductase(NAR), which was considered to be important to aerobic denitrification. pH and temperature influenced the nitrogen metabolism and changed the growth of G2. Its nitrogen removal rate can reach more than 90 percent in natural pH and low alkali environment(pH =7-9), but G2 can not grow in acidic conditions. G2 showed stronger growth and better denitrification at 25 °C - 35 °C. And the nitrate and nitrite removal rate were 59.1 mg N-NO3- removed h-1 g-1 dry wt of cells and 52.6 mg NO 2--N removed h-1 g-1 dry wt of cells in optimal cultivation pH (pH= 7) and the best growth temperature (T = 30 °C ).The data was significantly higher than that previously described in the literature. All the results revealed that the isolated G2 was able to rapid and effective aerobic nitrification. It had potential appliance value in actual natural pH and low alkali Nitrogen-Containing wastewater treating processes.
AB - A high efficient aerobic denitrifying bacterium(G2) capable of rapid removal of NO3- and NO2-was isolated from activated sludge. The purpose of this work was to study the characteristics, pH and temperature effects of the isolated bacterium. The morphology of the isolated aerobic denitrifier G2 was a short bacillus, with average size of (0.3-0.6)μm× (1.4-3.0) μm. According to the16S rDNA sequencing analysis, the isolated G2 was identified to be Paracoccus sp. The napA gene was cloned from G2. The gene was subunit of periplasmic nitrate reductase(NAR), which was considered to be important to aerobic denitrification. pH and temperature influenced the nitrogen metabolism and changed the growth of G2. Its nitrogen removal rate can reach more than 90 percent in natural pH and low alkali environment(pH =7-9), but G2 can not grow in acidic conditions. G2 showed stronger growth and better denitrification at 25 °C - 35 °C. And the nitrate and nitrite removal rate were 59.1 mg N-NO3- removed h-1 g-1 dry wt of cells and 52.6 mg NO 2--N removed h-1 g-1 dry wt of cells in optimal cultivation pH (pH= 7) and the best growth temperature (T = 30 °C ).The data was significantly higher than that previously described in the literature. All the results revealed that the isolated G2 was able to rapid and effective aerobic nitrification. It had potential appliance value in actual natural pH and low alkali Nitrogen-Containing wastewater treating processes.
KW - Aerobic denitrifying bacteria
KW - Denitrification rate
KW - Periplasmic nitrate reductase
KW - Temperature
KW - pH
UR - https://www.scopus.com/pages/publications/72749111635
U2 - 10.1109/ICBBE.2009.5162580
DO - 10.1109/ICBBE.2009.5162580
M3 - 会议稿件
AN - SCOPUS:72749111635
SN - 9781424429028
T3 - 3rd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2009
BT - 3rd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2009
T2 - 3rd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2009
Y2 - 11 June 2009 through 13 June 2009
ER -