Construction of carbon nanotube based nanoarchitectures for selective impedimetric detection of cancer cells in whole blood

Y. Liu; F. Zhu; W. Dan; Y. Fu; S. Liu

doi:10.1039/c4an00758a

Construction of carbon nanotube based nanoarchitectures for selective impedimetric detection of cancer cells in whole blood

Y. Liu^*
, F. Zhu
, W. Dan
, Y. Fu
, S. Liu

^*Corresponding author for this work

Faculty of Life Sciences and Medicine

Harbin Institute of Technology

Research output: Contribution to journal › Article › peer-review

52 Scopus citations

Abstract

A carbon nanotube (CNT) based nanoarchitecture is developed for rapid, sensitive and specific detection of cancer cells by using real time electrical impedance sensing. The sensor is constructed with carbon nanotube (CNT) multilayers and EpCAM (epithelial cell adhesion molecule) antibodies, which are assembled on an indium tin oxide (ITO) electrode surface. The binding of tumor cells to EpCAM antibodies causes increase of the electron-transfer resistance. The electrochemical impedance of the prepared biosensors is linear with the logarithm of concentration of the liver cancer cell line (HepG₂) within the concentration range of 10 to 10⁵ cells per mL. The detection limit for HepG₂ cells is 5 cells per mL. The proposed impedimetric sensing devices allow for sensitive and specific detection of cancer cells in whole-blood samples without any sample pretreatment steps.

Original language	English
Pages (from-to)	5086-5092
Number of pages	7
Journal	Analyst
Volume	139
Issue number	20
DOIs	https://doi.org/10.1039/c4an00758a
State	Published - 15 Sep 2014

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title = "Construction of carbon nanotube based nanoarchitectures for selective impedimetric detection of cancer cells in whole blood",

abstract = "A carbon nanotube (CNT) based nanoarchitecture is developed for rapid, sensitive and specific detection of cancer cells by using real time electrical impedance sensing. The sensor is constructed with carbon nanotube (CNT) multilayers and EpCAM (epithelial cell adhesion molecule) antibodies, which are assembled on an indium tin oxide (ITO) electrode surface. The binding of tumor cells to EpCAM antibodies causes increase of the electron-transfer resistance. The electrochemical impedance of the prepared biosensors is linear with the logarithm of concentration of the liver cancer cell line (HepG2) within the concentration range of 10 to 105 cells per mL. The detection limit for HepG2 cells is 5 cells per mL. The proposed impedimetric sensing devices allow for sensitive and specific detection of cancer cells in whole-blood samples without any sample pretreatment steps.",

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AU - Liu, Y.

AU - Zhu, F.

AU - Dan, W.

AU - Fu, Y.

AU - Liu, S.

PY - 2014/9/15

Y1 - 2014/9/15

N2 - A carbon nanotube (CNT) based nanoarchitecture is developed for rapid, sensitive and specific detection of cancer cells by using real time electrical impedance sensing. The sensor is constructed with carbon nanotube (CNT) multilayers and EpCAM (epithelial cell adhesion molecule) antibodies, which are assembled on an indium tin oxide (ITO) electrode surface. The binding of tumor cells to EpCAM antibodies causes increase of the electron-transfer resistance. The electrochemical impedance of the prepared biosensors is linear with the logarithm of concentration of the liver cancer cell line (HepG2) within the concentration range of 10 to 105 cells per mL. The detection limit for HepG2 cells is 5 cells per mL. The proposed impedimetric sensing devices allow for sensitive and specific detection of cancer cells in whole-blood samples without any sample pretreatment steps.

AB - A carbon nanotube (CNT) based nanoarchitecture is developed for rapid, sensitive and specific detection of cancer cells by using real time electrical impedance sensing. The sensor is constructed with carbon nanotube (CNT) multilayers and EpCAM (epithelial cell adhesion molecule) antibodies, which are assembled on an indium tin oxide (ITO) electrode surface. The binding of tumor cells to EpCAM antibodies causes increase of the electron-transfer resistance. The electrochemical impedance of the prepared biosensors is linear with the logarithm of concentration of the liver cancer cell line (HepG2) within the concentration range of 10 to 105 cells per mL. The detection limit for HepG2 cells is 5 cells per mL. The proposed impedimetric sensing devices allow for sensitive and specific detection of cancer cells in whole-blood samples without any sample pretreatment steps.

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DO - 10.1039/c4an00758a

M3 - 文章

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JF - Analyst

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ER -

Construction of carbon nanotube based nanoarchitectures for selective impedimetric detection of cancer cells in whole blood

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