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Configuration of nucleolar DNA in situ in nucleolus of Allium cepa cells

  • Wei Tao
  • , Jie He
  • , Mingda Jiao
  • , Mengyuan He
  • , Shui Hao*
  • *Corresponding author for this work
  • Peking University
  • Northeast Normal University

Research output: Contribution to journalArticlepeer-review

Abstract

The location and configuration of nucleolar DNA have not been determined for a long time. In this paper, we have observed the nucleolar ultrastructure and the character of nucleolar DNA in Allium cepa cells by conventional electron microscopy and the cytochemical NAMA-Ur DNA specific staining method. Furthermore, we have properly improved the NAMA-Ur method so as to analyze the location and configuration of nucleolar DNA in situ. Our results indicated that the nucleolar DNA in Allium cepa cells is mainly located at the border between fibrillar centers and dense fibrillar component, especially distributed in the configuration of encircling the fibrillar centers.

Original languageEnglish
Pages (from-to)62-66
Number of pages5
JournalChinese Science Bulletin
Volume46
Issue number1
DOIs
StatePublished - 2001
Externally publishedYes

Keywords

  • Allium cepa
  • Configuration of nucleolar DNA
  • NAMA-Ur method
  • Nucleolus

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