Comparison between INT and TTC assay to determine the dehydrogenase activity of flocs

Guochen Zheng; Feng Zhao; Hang Yang; Xue Lu; Jianzheng Li

doi:10.1109/ISWREP.2011.5893366

Comparison between INT and TTC assay to determine the dehydrogenase activity of flocs

Guochen Zheng^*
, Feng Zhao
, Hang Yang
, Xue Lu
, Jianzheng Li

^*Corresponding author for this work

School of Environment

Songliao River Basin Water Resources Protection Bureau

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

8 Scopus citations

Abstract

In order to evaluate the efficiency of anaerobic activated sludge in hydrogen-producing system, iodonitrotetrazolium chloride (INT) and triphenyl tetrazolium (TTC) assay were optimized for detecting the specific dehydrogenase activity of the flocs. The results showed that both INT and TTC assay under the optimum conditions could be used to determine dehydrogenase activity. In comparison with TTC assay, INT assay was more sensitive and precise. In the range between 3.5-12.5 g/L of sludge concentration (MLVSS), the INT assay are 2.8 mL reactant, 0.3 mL sludge sample, 1 mL 19.8 μmol/L INT solution, pH 5.0, an oscillating reaction at 45°C for 30 min, anhydrous ethanol as the extraction solvent and wavelength of 478 nm. The correlation coefficient between specific dehydrogenase activity and specific hydrogen production rate, R, was above 0.93, excellently reflecting the hydrogen-producing activity of flocs.

Original language	English
Title of host publication	ISWREP 2011 - Proceedings
Subtitle of host publication	of 2011 International Symposium on Water Resource and Environmental Protection
Pages	1690-1693
Number of pages	4
DOIs	https://doi.org/10.1109/ISWREP.2011.5893366
State	Published - 2011
Event	2011 International Symposium on Water Resource and Environmental Protection, ISWREP 2011 - Xi'an, China Duration: 20 May 2011 → 22 May 2011

Publication series

Name	ISWREP 2011 - Proceedings of 2011 International Symposium on Water Resource and Environmental Protection
Volume	3

Conference

Conference	2011 International Symposium on Water Resource and Environmental Protection, ISWREP 2011
Country/Territory	China
City	Xi'an
Period	20/05/11 → 22/05/11

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 7 Affordable and Clean Energy

Keywords

Anaerobi
Biohydrogen production
Dehydrogenase
Fermentation

Access to Document

10.1109/ISWREP.2011.5893366

Cite this

Zheng, G., Zhao, F., Yang, H., Lu, X., & Li, J. (2011). Comparison between INT and TTC assay to determine the dehydrogenase activity of flocs. In ISWREP 2011 - Proceedings: of 2011 International Symposium on Water Resource and Environmental Protection (pp. 1690-1693). Article 5893366 (ISWREP 2011 - Proceedings of 2011 International Symposium on Water Resource and Environmental Protection; Vol. 3). https://doi.org/10.1109/ISWREP.2011.5893366

Zheng, Guochen ; Zhao, Feng ; Yang, Hang et al. / Comparison between INT and TTC assay to determine the dehydrogenase activity of flocs. ISWREP 2011 - Proceedings: of 2011 International Symposium on Water Resource and Environmental Protection. 2011. pp. 1690-1693 (ISWREP 2011 - Proceedings of 2011 International Symposium on Water Resource and Environmental Protection).

@inproceedings{48bdfbde9ae444c6a727c6dd61b6016a,

title = "Comparison between INT and TTC assay to determine the dehydrogenase activity of flocs",

abstract = "In order to evaluate the efficiency of anaerobic activated sludge in hydrogen-producing system, iodonitrotetrazolium chloride (INT) and triphenyl tetrazolium (TTC) assay were optimized for detecting the specific dehydrogenase activity of the flocs. The results showed that both INT and TTC assay under the optimum conditions could be used to determine dehydrogenase activity. In comparison with TTC assay, INT assay was more sensitive and precise. In the range between 3.5-12.5 g/L of sludge concentration (MLVSS), the INT assay are 2.8 mL reactant, 0.3 mL sludge sample, 1 mL 19.8 μmol/L INT solution, pH 5.0, an oscillating reaction at 45°C for 30 min, anhydrous ethanol as the extraction solvent and wavelength of 478 nm. The correlation coefficient between specific dehydrogenase activity and specific hydrogen production rate, R, was above 0.93, excellently reflecting the hydrogen-producing activity of flocs.",

keywords = "Anaerobi, Biohydrogen production, Dehydrogenase, Fermentation",

author = "Guochen Zheng and Feng Zhao and Hang Yang and Xue Lu and Jianzheng Li",

year = "2011",

doi = "10.1109/ISWREP.2011.5893366",

language = "英语",

isbn = "9781612843377",

series = "ISWREP 2011 - Proceedings of 2011 International Symposium on Water Resource and Environmental Protection",

pages = "1690--1693",

booktitle = "ISWREP 2011 - Proceedings",

note = "2011 International Symposium on Water Resource and Environmental Protection, ISWREP 2011 ; Conference date: 20-05-2011 Through 22-05-2011",

}

Zheng, G, Zhao, F, Yang, H, Lu, X & Li, J 2011, Comparison between INT and TTC assay to determine the dehydrogenase activity of flocs. in ISWREP 2011 - Proceedings: of 2011 International Symposium on Water Resource and Environmental Protection., 5893366, ISWREP 2011 - Proceedings of 2011 International Symposium on Water Resource and Environmental Protection, vol. 3, pp. 1690-1693, 2011 International Symposium on Water Resource and Environmental Protection, ISWREP 2011, Xi'an, China, 20/05/11. https://doi.org/10.1109/ISWREP.2011.5893366

Comparison between INT and TTC assay to determine the dehydrogenase activity of flocs. / Zheng, Guochen; Zhao, Feng; Yang, Hang et al.
ISWREP 2011 - Proceedings: of 2011 International Symposium on Water Resource and Environmental Protection. 2011. p. 1690-1693 5893366 (ISWREP 2011 - Proceedings of 2011 International Symposium on Water Resource and Environmental Protection; Vol. 3).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Comparison between INT and TTC assay to determine the dehydrogenase activity of flocs

AU - Zheng, Guochen

AU - Zhao, Feng

AU - Yang, Hang

AU - Lu, Xue

AU - Li, Jianzheng

PY - 2011

Y1 - 2011

N2 - In order to evaluate the efficiency of anaerobic activated sludge in hydrogen-producing system, iodonitrotetrazolium chloride (INT) and triphenyl tetrazolium (TTC) assay were optimized for detecting the specific dehydrogenase activity of the flocs. The results showed that both INT and TTC assay under the optimum conditions could be used to determine dehydrogenase activity. In comparison with TTC assay, INT assay was more sensitive and precise. In the range between 3.5-12.5 g/L of sludge concentration (MLVSS), the INT assay are 2.8 mL reactant, 0.3 mL sludge sample, 1 mL 19.8 μmol/L INT solution, pH 5.0, an oscillating reaction at 45°C for 30 min, anhydrous ethanol as the extraction solvent and wavelength of 478 nm. The correlation coefficient between specific dehydrogenase activity and specific hydrogen production rate, R, was above 0.93, excellently reflecting the hydrogen-producing activity of flocs.

AB - In order to evaluate the efficiency of anaerobic activated sludge in hydrogen-producing system, iodonitrotetrazolium chloride (INT) and triphenyl tetrazolium (TTC) assay were optimized for detecting the specific dehydrogenase activity of the flocs. The results showed that both INT and TTC assay under the optimum conditions could be used to determine dehydrogenase activity. In comparison with TTC assay, INT assay was more sensitive and precise. In the range between 3.5-12.5 g/L of sludge concentration (MLVSS), the INT assay are 2.8 mL reactant, 0.3 mL sludge sample, 1 mL 19.8 μmol/L INT solution, pH 5.0, an oscillating reaction at 45°C for 30 min, anhydrous ethanol as the extraction solvent and wavelength of 478 nm. The correlation coefficient between specific dehydrogenase activity and specific hydrogen production rate, R, was above 0.93, excellently reflecting the hydrogen-producing activity of flocs.

KW - Anaerobi

KW - Biohydrogen production

KW - Dehydrogenase

KW - Fermentation

UR - https://www.scopus.com/pages/publications/79960705305

U2 - 10.1109/ISWREP.2011.5893366

DO - 10.1109/ISWREP.2011.5893366

M3 - 会议稿件

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SN - 9781612843377

T3 - ISWREP 2011 - Proceedings of 2011 International Symposium on Water Resource and Environmental Protection

SP - 1690

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BT - ISWREP 2011 - Proceedings

T2 - 2011 International Symposium on Water Resource and Environmental Protection, ISWREP 2011

Y2 - 20 May 2011 through 22 May 2011

ER -

Zheng G, Zhao F, Yang H, Lu X, Li J. Comparison between INT and TTC assay to determine the dehydrogenase activity of flocs. In ISWREP 2011 - Proceedings: of 2011 International Symposium on Water Resource and Environmental Protection. 2011. p. 1690-1693. 5893366. (ISWREP 2011 - Proceedings of 2011 International Symposium on Water Resource and Environmental Protection). doi: 10.1109/ISWREP.2011.5893366

Comparison between INT and TTC assay to determine the dehydrogenase activity of flocs

Abstract

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UN SDGs

Keywords

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