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Chitosan functionalized CuS nanoparticles boots gene transfection via photothermal effect

  • Li Lin
  • , Xiaoda Li
  • , Yongbo Yang
  • , Lijia Jing
  • , Xiuli Yue*
  • , Xuzhu Chen
  • , Zhifei Dai
  • *Corresponding author for this work
  • Harbin Institute of Technology
  • Capital Medical University
  • Peking University

Research output: Contribution to journalArticlepeer-review

Abstract

The chitosan functionalized CuS nanoparticles around 15 nm were successfully engineered by employing hydrothermal method. The modification of CuS nanoparticles with chitosan conduced to higher physiological stability and biocompatibility. The utilization of CuS@CS NPs in combination with external near infrared (NIR) laser irradiation could enhance gene transfection efficiency due to photothermal effect. The gene transfection efficiency of CuS@CS NPs found to increase from 5.05 ± 0.54% (0 min) to 23.47 ± 1.27% (10 min), significantly higher than the free polyethylenimine (18.15 ± 1.03%). Therefore CuS@CS NPs showed great capability to control gene delivery by an external NIR laser irradiation and enhance the gene transfection efficiency and specificity because of convenient preparation, stabilized optical properties, excellent photothermal effect and good biocompatibility. It encourages further exploration of the CuS@CS NPs as a photocontrollable nanovector for combined photothermal and gene therapy, as well as image guided therapy.

Original languageEnglish
Pages (from-to)334-341
Number of pages8
JournalCurrent Drug Delivery
Volume13
DOIs
StatePublished - 2016

Keywords

  • Biocompatibility
  • Chitosan
  • Copper sulfide
  • Gene transfection
  • Photocontrollable nanovector
  • Photothermal effect

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