Characterizing the fluid dynamics of the inverted frustoconical shaking bioreactor

Likuan Zhu; Xueting Zhang; Kai Cheng; Zhonghua Lv; Lei Zhang; Qingyong Meng; Shujie Yuan; Boyan Song; Zhenlong Wang

doi:10.1002/btpr.2602

Characterizing the fluid dynamics of the inverted frustoconical shaking bioreactor

Likuan Zhu
, Xueting Zhang
, Kai Cheng
, Zhonghua Lv
, Lei Zhang
, Qingyong Meng
, Shujie Yuan
, Boyan Song
, Zhenlong Wang^*

^*Corresponding author for this work

School of Mechatronics Engineering, Harbin Institute of Technology
Pharmaceutical research center of Harbin Bioengineering Corporation

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

The authors conducted a three-dimensional computational fluid dynamics (CFD) simulation to calculate the flow field in the inverted frustoconical shaking bioreactor with 5 L working volume (IFSB-5L). The CFD models were established for the IFSB-5L at different operating conditions (different shaking speeds and filling volumes) and validated by comparison of the liquid height distribution in the agitated IFSB-5L. The “out of phase” operating conditions were characterized by analyzing the flow field in the IFSB-5L at different filling volumes and shaking speeds. The values of volumetric power consumption (P/V_L) and volumetric mass transfer coefficient (k_La) were determined from simulated and experimental results, respectively. Finally, the operating condition effect on P/V_L and k_La was investigated.

Original language	English
Pages (from-to)	478-485
Number of pages	8
Journal	Biotechnology Progress
Volume	34
Issue number	2
DOIs	https://doi.org/10.1002/btpr.2602
State	Published - 1 Mar 2018
Externally published	Yes

Keywords

computational fluid dynamics
operating condition
orbitally shaken bioreactor
out of phase

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10.1002/btpr.2602

Cite this

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title = "Characterizing the fluid dynamics of the inverted frustoconical shaking bioreactor",

abstract = "The authors conducted a three-dimensional computational fluid dynamics (CFD) simulation to calculate the flow field in the inverted frustoconical shaking bioreactor with 5 L working volume (IFSB-5L). The CFD models were established for the IFSB-5L at different operating conditions (different shaking speeds and filling volumes) and validated by comparison of the liquid height distribution in the agitated IFSB-5L. The “out of phase” operating conditions were characterized by analyzing the flow field in the IFSB-5L at different filling volumes and shaking speeds. The values of volumetric power consumption (P/VL) and volumetric mass transfer coefficient (kLa) were determined from simulated and experimental results, respectively. Finally, the operating condition effect on P/VL and kLa was investigated.",

keywords = "computational fluid dynamics, operating condition, orbitally shaken bioreactor, out of phase",

author = "Likuan Zhu and Xueting Zhang and Kai Cheng and Zhonghua Lv and Lei Zhang and Qingyong Meng and Shujie Yuan and Boyan Song and Zhenlong Wang",

note = "Publisher Copyright: {\textcopyright} 2018 American Institute of Chemical Engineers",

year = "2018",

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doi = "10.1002/btpr.2602",

language = "英语",

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journal = "Biotechnology Progress",

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TY - JOUR

T1 - Characterizing the fluid dynamics of the inverted frustoconical shaking bioreactor

AU - Zhu, Likuan

AU - Zhang, Xueting

AU - Cheng, Kai

AU - Lv, Zhonghua

AU - Zhang, Lei

AU - Meng, Qingyong

AU - Yuan, Shujie

AU - Song, Boyan

AU - Wang, Zhenlong

PY - 2018/3/1

Y1 - 2018/3/1

N2 - The authors conducted a three-dimensional computational fluid dynamics (CFD) simulation to calculate the flow field in the inverted frustoconical shaking bioreactor with 5 L working volume (IFSB-5L). The CFD models were established for the IFSB-5L at different operating conditions (different shaking speeds and filling volumes) and validated by comparison of the liquid height distribution in the agitated IFSB-5L. The “out of phase” operating conditions were characterized by analyzing the flow field in the IFSB-5L at different filling volumes and shaking speeds. The values of volumetric power consumption (P/VL) and volumetric mass transfer coefficient (kLa) were determined from simulated and experimental results, respectively. Finally, the operating condition effect on P/VL and kLa was investigated.

AB - The authors conducted a three-dimensional computational fluid dynamics (CFD) simulation to calculate the flow field in the inverted frustoconical shaking bioreactor with 5 L working volume (IFSB-5L). The CFD models were established for the IFSB-5L at different operating conditions (different shaking speeds and filling volumes) and validated by comparison of the liquid height distribution in the agitated IFSB-5L. The “out of phase” operating conditions were characterized by analyzing the flow field in the IFSB-5L at different filling volumes and shaking speeds. The values of volumetric power consumption (P/VL) and volumetric mass transfer coefficient (kLa) were determined from simulated and experimental results, respectively. Finally, the operating condition effect on P/VL and kLa was investigated.

KW - computational fluid dynamics

KW - operating condition

KW - orbitally shaken bioreactor

KW - out of phase

UR - https://www.scopus.com/pages/publications/85040740286

U2 - 10.1002/btpr.2602

DO - 10.1002/btpr.2602

M3 - 文章

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AN - SCOPUS:85040740286

SN - 8756-7938

VL - 34

SP - 478

EP - 485

JO - Biotechnology Progress

JF - Biotechnology Progress

IS - 2

ER -

Characterizing the fluid dynamics of the inverted frustoconical shaking bioreactor

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Keywords

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