Bioabatement with hemicellulase supplementation to reduce enzymatic hydrolysis inhibitors

Guangli Cao; Eduardo Ximenes; Nancy N. Nichols; Sarah E. Frazer; Daehwan Kim; Michael A. Cotta; Michael Ladisch

doi:10.1016/j.biortech.2015.04.064

Bioabatement with hemicellulase supplementation to reduce enzymatic hydrolysis inhibitors

Guangli Cao
, Eduardo Ximenes
, Nancy N. Nichols
, Sarah E. Frazer
, Daehwan Kim
, Michael A. Cotta
, Michael Ladisch^*

^*Corresponding author for this work

School of Environment

Purdue University
School of Life Science and Technology, Harbin Institute of Technology
United States Department of Agriculture

Research output: Contribution to journal › Article › peer-review

47 Scopus citations

Abstract

A stepwise removal of inhibitory compounds by bioabatement combined with hemicellulase supplementation was conducted to enhance cellulose hydrolysis of liquid hot water-pretreated corn stover. Results showed that the fungus Coniochaeta ligniaria NRRL30616 eliminated most of the enzyme and fermentation inhibitors from liquid hot water-pretreated corn stover hydrolysates. Moreover, addition of hemicellulases after bioabatement and before enzymatic hydrolysis of cellulose achieved 20% higher glucose yields compared to non-treated samples. This work presents the mechanisms by which supplementation of the fungus with hemicellulase enzymes enables maximal conversion, and confirms the inhibitory effect of xylo-oligosaccharides in corn stover hydrolysates once the dominant inhibitory effect of phenolic compounds is removed.

Original language	English
Pages (from-to)	412-415
Number of pages	4
Journal	Bioresource Technology
Volume	190
DOIs	https://doi.org/10.1016/j.biortech.2015.04.064
State	Published - 1 Aug 2015

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 7 Affordable and Clean Energy

Keywords

Bioabatement
Enzymatic hydrolysis
Hemicellulase supplementation
Inhibitors
Liquid hot water biomass pretreatment

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10.1016/j.biortech.2015.04.064

Cite this

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title = "Bioabatement with hemicellulase supplementation to reduce enzymatic hydrolysis inhibitors",

abstract = "A stepwise removal of inhibitory compounds by bioabatement combined with hemicellulase supplementation was conducted to enhance cellulose hydrolysis of liquid hot water-pretreated corn stover. Results showed that the fungus Coniochaeta ligniaria NRRL30616 eliminated most of the enzyme and fermentation inhibitors from liquid hot water-pretreated corn stover hydrolysates. Moreover, addition of hemicellulases after bioabatement and before enzymatic hydrolysis of cellulose achieved 20\% higher glucose yields compared to non-treated samples. This work presents the mechanisms by which supplementation of the fungus with hemicellulase enzymes enables maximal conversion, and confirms the inhibitory effect of xylo-oligosaccharides in corn stover hydrolysates once the dominant inhibitory effect of phenolic compounds is removed.",

keywords = "Bioabatement, Enzymatic hydrolysis, Hemicellulase supplementation, Inhibitors, Liquid hot water biomass pretreatment",

author = "Guangli Cao and Eduardo Ximenes and Nichols, \{Nancy N.\} and Frazer, \{Sarah E.\} and Daehwan Kim and Cotta, \{Michael A.\} and Michael Ladisch",

note = "Publisher Copyright: {\textcopyright} 2015 Elsevier Ltd.",

year = "2015",

month = aug,

day = "1",

doi = "10.1016/j.biortech.2015.04.064",

language = "英语",

volume = "190",

pages = "412--415",

journal = "Bioresource Technology",

issn = "0960-8524",

publisher = "Elsevier Ltd",

}

TY - JOUR

T1 - Bioabatement with hemicellulase supplementation to reduce enzymatic hydrolysis inhibitors

AU - Cao, Guangli

AU - Ximenes, Eduardo

AU - Nichols, Nancy N.

AU - Frazer, Sarah E.

AU - Kim, Daehwan

AU - Cotta, Michael A.

AU - Ladisch, Michael

PY - 2015/8/1

Y1 - 2015/8/1

N2 - A stepwise removal of inhibitory compounds by bioabatement combined with hemicellulase supplementation was conducted to enhance cellulose hydrolysis of liquid hot water-pretreated corn stover. Results showed that the fungus Coniochaeta ligniaria NRRL30616 eliminated most of the enzyme and fermentation inhibitors from liquid hot water-pretreated corn stover hydrolysates. Moreover, addition of hemicellulases after bioabatement and before enzymatic hydrolysis of cellulose achieved 20% higher glucose yields compared to non-treated samples. This work presents the mechanisms by which supplementation of the fungus with hemicellulase enzymes enables maximal conversion, and confirms the inhibitory effect of xylo-oligosaccharides in corn stover hydrolysates once the dominant inhibitory effect of phenolic compounds is removed.

AB - A stepwise removal of inhibitory compounds by bioabatement combined with hemicellulase supplementation was conducted to enhance cellulose hydrolysis of liquid hot water-pretreated corn stover. Results showed that the fungus Coniochaeta ligniaria NRRL30616 eliminated most of the enzyme and fermentation inhibitors from liquid hot water-pretreated corn stover hydrolysates. Moreover, addition of hemicellulases after bioabatement and before enzymatic hydrolysis of cellulose achieved 20% higher glucose yields compared to non-treated samples. This work presents the mechanisms by which supplementation of the fungus with hemicellulase enzymes enables maximal conversion, and confirms the inhibitory effect of xylo-oligosaccharides in corn stover hydrolysates once the dominant inhibitory effect of phenolic compounds is removed.

KW - Bioabatement

KW - Enzymatic hydrolysis

KW - Hemicellulase supplementation

KW - Inhibitors

KW - Liquid hot water biomass pretreatment

UR - https://www.scopus.com/pages/publications/84930181803

U2 - 10.1016/j.biortech.2015.04.064

DO - 10.1016/j.biortech.2015.04.064

M3 - 文章

C2 - 25958134

AN - SCOPUS:84930181803

SN - 0960-8524

VL - 190

SP - 412

EP - 415

JO - Bioresource Technology

JF - Bioresource Technology

ER -

Bioabatement with hemicellulase supplementation to reduce enzymatic hydrolysis inhibitors

Abstract

UN SDGs

Keywords

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