Bead-based microfluidic RT-QPCR analysis of single cancer cells

H. Sun; T. Olsen; J. Zhu; B. Ponnaiya; S. Amundson; D. Brenner; J. Tao; Q. Lin

Bead-based microfluidic RT-QPCR analysis of single cancer cells

H. Sun
, T. Olsen
, J. Zhu
, B. Ponnaiya
, S. Amundson
, D. Brenner
, J. Tao
, Q. Lin^*

^*Corresponding author for this work

School of Mechatronics Engineering

Columbia University

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

Single-cell microfluidic RT-qPCR can enable gene expression assays of small volumes, with efficient thermal cycling, and reduced costs. Such devices currently use solution-based methods, which, while well established, do not allow efficient manipulation of samples and reagents or retrieval of reaction products for real-time single-cell genetic analysis. Employing a novel microbead-based approach and for the first time, we present a microchip that integrates all steps for genetic analysis of an individual cell. The effects of the chemotherapy drug (MMS) on the induction of CDKN1A in single human cancer cells (MCF-7) were assayed, demonstrating the potential of the device for single-cell analysis.

Original language	English
Title of host publication	18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
Publisher	Chemical and Biological Microsystems Society
Pages	811-813
Number of pages	3
ISBN (Electronic)	9780979806476
State	Published - 2014
Event	18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 - San Antonio, United States Duration: 26 Oct 2014 → 30 Oct 2014

Publication series

Name	18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014

Conference

Conference	18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
Country/Territory	United States
City	San Antonio
Period	26/10/14 → 30/10/14

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Bead-based
Microfluidics
RT-qPCR
Single-cell

Cite this

Sun, H., Olsen, T., Zhu, J., Ponnaiya, B., Amundson, S., Brenner, D., Tao, J., & Lin, Q. (2014). Bead-based microfluidic RT-QPCR analysis of single cancer cells. In 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 (pp. 811-813). (18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014). Chemical and Biological Microsystems Society.

Sun, H. ; Olsen, T. ; Zhu, J. et al. / Bead-based microfluidic RT-QPCR analysis of single cancer cells. 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. Chemical and Biological Microsystems Society, 2014. pp. 811-813 (18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014).

@inproceedings{719ca074001542af972c74c4a50dea18,

title = "Bead-based microfluidic RT-QPCR analysis of single cancer cells",

abstract = "Single-cell microfluidic RT-qPCR can enable gene expression assays of small volumes, with efficient thermal cycling, and reduced costs. Such devices currently use solution-based methods, which, while well established, do not allow efficient manipulation of samples and reagents or retrieval of reaction products for real-time single-cell genetic analysis. Employing a novel microbead-based approach and for the first time, we present a microchip that integrates all steps for genetic analysis of an individual cell. The effects of the chemotherapy drug (MMS) on the induction of CDKN1A in single human cancer cells (MCF-7) were assayed, demonstrating the potential of the device for single-cell analysis.",

keywords = "Bead-based, Microfluidics, RT-qPCR, Single-cell",

author = "H. Sun and T. Olsen and J. Zhu and B. Ponnaiya and S. Amundson and D. Brenner and J. Tao and Q. Lin",

note = "Publisher Copyright: {\textcopyright} 14CBMS.; 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 ; Conference date: 26-10-2014 Through 30-10-2014",

year = "2014",

language = "英语",

series = "18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014",

publisher = "Chemical and Biological Microsystems Society",

pages = "811--813",

booktitle = "18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014",

}

Sun, H, Olsen, T, Zhu, J, Ponnaiya, B, Amundson, S, Brenner, D, Tao, J & Lin, Q 2014, Bead-based microfluidic RT-QPCR analysis of single cancer cells. in 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014, Chemical and Biological Microsystems Society, pp. 811-813, 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014, San Antonio, United States, 26/10/14.

Bead-based microfluidic RT-QPCR analysis of single cancer cells. / Sun, H.; Olsen, T.; Zhu, J. et al.
18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. Chemical and Biological Microsystems Society, 2014. p. 811-813 (18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Bead-based microfluidic RT-QPCR analysis of single cancer cells

AU - Sun, H.

AU - Olsen, T.

AU - Zhu, J.

AU - Ponnaiya, B.

AU - Amundson, S.

AU - Brenner, D.

AU - Tao, J.

AU - Lin, Q.

PY - 2014

Y1 - 2014

N2 - Single-cell microfluidic RT-qPCR can enable gene expression assays of small volumes, with efficient thermal cycling, and reduced costs. Such devices currently use solution-based methods, which, while well established, do not allow efficient manipulation of samples and reagents or retrieval of reaction products for real-time single-cell genetic analysis. Employing a novel microbead-based approach and for the first time, we present a microchip that integrates all steps for genetic analysis of an individual cell. The effects of the chemotherapy drug (MMS) on the induction of CDKN1A in single human cancer cells (MCF-7) were assayed, demonstrating the potential of the device for single-cell analysis.

AB - Single-cell microfluidic RT-qPCR can enable gene expression assays of small volumes, with efficient thermal cycling, and reduced costs. Such devices currently use solution-based methods, which, while well established, do not allow efficient manipulation of samples and reagents or retrieval of reaction products for real-time single-cell genetic analysis. Employing a novel microbead-based approach and for the first time, we present a microchip that integrates all steps for genetic analysis of an individual cell. The effects of the chemotherapy drug (MMS) on the induction of CDKN1A in single human cancer cells (MCF-7) were assayed, demonstrating the potential of the device for single-cell analysis.

KW - Bead-based

KW - Microfluidics

KW - RT-qPCR

KW - Single-cell

UR - https://www.scopus.com/pages/publications/84941634818

M3 - 会议稿件

AN - SCOPUS:84941634818

T3 - 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014

SP - 811

EP - 813

BT - 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014

PB - Chemical and Biological Microsystems Society

T2 - 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014

Y2 - 26 October 2014 through 30 October 2014

ER -

Sun H, Olsen T, Zhu J, Ponnaiya B, Amundson S, Brenner D et al. Bead-based microfluidic RT-QPCR analysis of single cancer cells. In 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. Chemical and Biological Microsystems Society. 2014. p. 811-813. (18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014).

Bead-based microfluidic RT-QPCR analysis of single cancer cells

Abstract

Publication series

Conference

UN SDGs

Keywords

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