Applying 454 pyrosequencing technology to analyze bacterial communities with operation time in cattle dung-fed microbial fuel cell

Cattle dung was used as a fermentable substrate to generate power during acidogenesis. The massively parallel sequencing technology, 454 pyrosequencing technique was applied to analyze the microbial community on anode biofilm during operation time. The dominant phyla in cattle dung were Firmicutes (66.4%), Proteobacteria (15.2%) and Bacteroidetes (11.3%). Notably, the most dominant bacteria were Proteobacteria on day 40, 60 and 80 of operation, which was a major divergence from cattle dung and accounted for 55.5%, 52.9% and 49.9%, respectively. At genera level, the dominant community (relative abundances > 4.0%) were genus Sporacetigenium (25.83%), Anaerobacter (4.93%) and Turicibacter (4.61%) in cattle dung. Genus Acinetobacter were predominant on day 40, 60 and 80, accounted for 20.52%, 16.14 % and 8.14%, respectively.