Application of PCR-denaturing gradient gel electrophoresis (PCR-DGGE) in environmental microbial ecology

Guobin Shan; Wenbiao Jin; Jikan Lin; Xinhui Xing

Application of PCR-denaturing gradient gel electrophoresis (PCR-DGGE) in environmental microbial ecology

Guobin Shan^*
, Wenbiao Jin
, Jikan Lin
, Xinhui Xing

^*Corresponding author for this work

Baolimei Chemical Engineering Co., Ltd.
Tsinghua University
Harbin Institute of Technology Shenzhen
Bio-Treat Technology Limited

Research output: Contribution to journal › Review article › peer-review

2 Scopus citations

Abstract

PCR-denaturing gradient gel electrophoresis (PCR-DGGE) has the advantages of high reliability, good repetition and easy operation, and been widely used in environmental ecology to analyze the diversity and dynamics of microbial communities, and to monitor functional bacteria. This paper introduced the principles and methods of PCR-DGGE, reviewed its application in environmental ecological study, and analyzed its potential application prospects and limitations.

Original language	English
Pages (from-to)	1257-1264
Number of pages	8
Journal	Chinese Journal of Ecology
Volume	25
Issue number	10
State	Published - Oct 2006
Externally published	Yes

Keywords

Activated sludge
Biofilm
Microbial community
Nitrifying-denitrifying bacteria
PCR-DGGE
Sulfate-reducing bacteria
Wastewater bio-treatment

Cite this

@article{693d0be1ebaf40afaf2ad04d1ccc9b44,

title = "Application of PCR-denaturing gradient gel electrophoresis (PCR-DGGE) in environmental microbial ecology",

abstract = "PCR-denaturing gradient gel electrophoresis (PCR-DGGE) has the advantages of high reliability, good repetition and easy operation, and been widely used in environmental ecology to analyze the diversity and dynamics of microbial communities, and to monitor functional bacteria. This paper introduced the principles and methods of PCR-DGGE, reviewed its application in environmental ecological study, and analyzed its potential application prospects and limitations.",

keywords = "Activated sludge, Biofilm, Microbial community, Nitrifying-denitrifying bacteria, PCR-DGGE, Sulfate-reducing bacteria, Wastewater bio-treatment",

author = "Guobin Shan and Wenbiao Jin and Jikan Lin and Xinhui Xing",

year = "2006",

month = oct,

language = "英语",

volume = "25",

pages = "1257--1264",

journal = "Chinese Journal of Ecology",

issn = "1000-4890",

publisher = "Ecological Society of China",

number = "10",

}

TY - JOUR

T1 - Application of PCR-denaturing gradient gel electrophoresis (PCR-DGGE) in environmental microbial ecology

AU - Shan, Guobin

AU - Jin, Wenbiao

AU - Lin, Jikan

AU - Xing, Xinhui

PY - 2006/10

Y1 - 2006/10

N2 - PCR-denaturing gradient gel electrophoresis (PCR-DGGE) has the advantages of high reliability, good repetition and easy operation, and been widely used in environmental ecology to analyze the diversity and dynamics of microbial communities, and to monitor functional bacteria. This paper introduced the principles and methods of PCR-DGGE, reviewed its application in environmental ecological study, and analyzed its potential application prospects and limitations.

AB - PCR-denaturing gradient gel electrophoresis (PCR-DGGE) has the advantages of high reliability, good repetition and easy operation, and been widely used in environmental ecology to analyze the diversity and dynamics of microbial communities, and to monitor functional bacteria. This paper introduced the principles and methods of PCR-DGGE, reviewed its application in environmental ecological study, and analyzed its potential application prospects and limitations.

KW - Activated sludge

KW - Biofilm

KW - Microbial community

KW - Nitrifying-denitrifying bacteria

KW - PCR-DGGE

KW - Sulfate-reducing bacteria

KW - Wastewater bio-treatment

UR - https://www.scopus.com/pages/publications/33845507428

M3 - 文献综述

AN - SCOPUS:33845507428

SN - 1000-4890

VL - 25

SP - 1257

EP - 1264

JO - Chinese Journal of Ecology

JF - Chinese Journal of Ecology

IS - 10

ER -

Application of PCR-denaturing gradient gel electrophoresis (PCR-DGGE) in environmental microbial ecology

Abstract

Keywords

Other files and links

Fingerprint

Cite this