Application of microfluidics to study stem cell dynamics

Huaying Chen; Robert E. Nordon

doi:10.1007/978-1-62703-417-3_19

Application of microfluidics to study stem cell dynamics

Huaying Chen
, Robert E. Nordon^*

^*Corresponding author for this work

University of New South Wales
Shandong University

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

4 Scopus citations

Abstract

Stem cell fate is directed by a complex chemical and mechanical microenvironment composed of secreted factors, extracellular matrix, and direct interactions with other cells. These signals ultimately control stem cell renewal and lineage fate in a developmental context. It may be possible to dissect the role of specific signaling pathways by precise control of microenvironment. However, traditional flask cell culture methods are unable to control microenvironment at microscale. Microfluidic platforms have the potential of mimicking the signals that direct stem cell fate by precise control of the chemical and mechanical milieu of cells at microscale. Furthermore, so called lab-on-a-chip technologies can increase research throughput by cost-effect automation of multiple parallel microscale cultures. This chapter will reveal how microfluidics and lab-on-a-chip technologies can be applied to the study of stem cell dynamics.

Original language	English
Title of host publication	Emerging Trends in Cell and Gene Therapy
Publisher	Humana Press Inc.
Pages	435-470
Number of pages	36
ISBN (Electronic)	9781627034173
ISBN (Print)	1627034161, 9781627034166
DOIs	https://doi.org/10.1007/978-1-62703-417-3_19
State	Published - 1 Dec 2013
Externally published	Yes

Keywords

Computational fluid dynamics
Lab-on-a-chip
Microfluidics
Photolithography
Stem cell dynamics

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10.1007/978-1-62703-417-3_19

Cite this

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AB - Stem cell fate is directed by a complex chemical and mechanical microenvironment composed of secreted factors, extracellular matrix, and direct interactions with other cells. These signals ultimately control stem cell renewal and lineage fate in a developmental context. It may be possible to dissect the role of specific signaling pathways by precise control of microenvironment. However, traditional flask cell culture methods are unable to control microenvironment at microscale. Microfluidic platforms have the potential of mimicking the signals that direct stem cell fate by precise control of the chemical and mechanical milieu of cells at microscale. Furthermore, so called lab-on-a-chip technologies can increase research throughput by cost-effect automation of multiple parallel microscale cultures. This chapter will reveal how microfluidics and lab-on-a-chip technologies can be applied to the study of stem cell dynamics.

KW - Computational fluid dynamics

KW - Lab-on-a-chip

KW - Microfluidics

KW - Photolithography

KW - Stem cell dynamics

UR - https://www.scopus.com/pages/publications/84929893751

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SP - 435

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BT - Emerging Trends in Cell and Gene Therapy

PB - Humana Press Inc.

ER -

Application of microfluidics to study stem cell dynamics

Abstract

Keywords

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