Analysis of microbial community dynamics and population stability in BEAC procedure

To study microbial community dynamics and population stability on the bioenhancement activated carbon (BEAC), the samples were taken from different position of the carbon column at different operation time. The Genomic DNA of the microbes on the activated carbon was released by breaking the bacterial cells. After purification of the genomic DNA, the V3 region of genomic 16S rDNA was amplified by using specific primers (F357-GC and R518). The polymerase chain reaction (PCR) products were about 250 bp and then separated by parallel denaturing gradient gel electrophoresis (DGGE). The profile of DGGE showed that the structure, categories and number of microbial community were changing dynamically during running of the reactor. The categories and number of the inbreaking bacteria were reducing continuously. 5 artificial enhanced and immobilized bacteria were existing all through and distributed differently in the carbon column. Further more, they became dominant community after dynamic succession and community structure tended to be stable. The stability of the immobilized bacteria ensured the stability of operation and removal effect of BEAC procedure.