Analysis of bio-hydrogen production capacity of the cellulose degradation by cellulolyticum sp.X₉ and ethanoigenensharbinense B₄₉

As advanced renewable energy, hydrogen is attracted more and more attention. Corn stalks are produced in huge amount throughout the world, which could serve as ideal raw materials for fermentative bio-hydrogen production. In this study, Cellulolyticum sp.X₉ and Ethanoigenens harbinense B₄₉, anaerobic high efficiency cellulose degrading and hydrogen producing bacterium, were isolated from a continuous flow H₂ producing reactor (ZL92114474.1). The compound bacteria was more preponderant than X₉ or B₄₉ for fermentative bio-hydrogen production. The conditions of fermentative bio-hydrogen production are: Inoculum ratio 1:1, inoculum size 10%, temperature 40.0°C, mixed culture time 24 h. Experimental results showed that compound strain X₉ and B₄₉ has high hydrogen yield (Y_H2) of 1530 ml/L and corn stalks degradation degree of 61.8% feeding with pre-treatment corn stalks. This indicated that compound bacteria Cellulolyticum sp.X₉ and Ethanoigenens harbinense B₄₉ has great potential for fermentative H₂ production from corn stalks after effective pretreatment.