@inproceedings{f3d72404d0c84b2591f799bb51548cb6,
title = "An effective combined-enhancer for the PCR amplification of GC-rich longer DNA sequences",
abstract = "DNA manipulation technology is facing more challenges in the postgenomics era. The amplification of GC-rich longer DNA fragments PCR more difficult than others . through more and more enhancers have been investigated for their potential implications in developing better gene technology. In this study, we reported a beneficial effect of combined-enhancer in enhancing the specificity and total efficiency of GC-rich (GC\%≥65\%) longer DNA fragments PCR. Combined-enhancer is a concentration-dependent combination of 5.5M betaine ,0.5u pfu enzyme, 1.5mM the mixture of 1,2-propylene glycol and ethylene glycol (the ration of 1,2-propylene glycol and ethylene glycol = 1:1)in 25 μL PCR condition. The combined-enhancer broadly enhanced the quantitative and/or qualitative output of GC-rich?GC\%≥65?longer DNA fragments PCRs.",
keywords = "Combined-enhancer, High GC, Longer fragment, PCR",
author = "Fang Liu and Wenze Zhu and Zhizhou Zhang",
year = "2009",
doi = "10.1109/ICBBE.2009.5162313",
language = "英语",
isbn = "9781424429028",
series = "3rd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2009",
booktitle = "3rd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2009",
note = "3rd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2009 ; Conference date: 11-06-2009 Through 13-06-2009",
}