Airy beam modulation and its application in 3D microscopic Imaging

Airy beam has the characteristics of non-diffraction, self-healing and self-acceleration, which has attracted much attention in the fields of optical manipulation, nonlinear optics and optical imaging. This paper introduces the method of generating and controlling Airy beam by using 4f system constructed by a spatial light modulator. By adjusting the self-bending propagation point spread function of the Airy beam, the Airy beam three-dimensional reconstruction microscope is cesigned and realized, and the imaging performance of the microscope is stuilied. Using a 40×/0.75 NA objective lens, the lateral resolution is about 0.5 μm, and the axial resolution is about 1.5 μm, and 3D non-scanning two-color imaging of kidney tubules is realized in mouse kidney cells with 10 μm depth.