Achieving efficient anammox contribution and the enrichment of functional bacteria in partial denitrification/anammox system: Performance, microbial evolution and correlation analysis

Zilong Hou; Wenyi Dong; Yanchen Li; Shuo Chen; Huaguang Liu; Qi Han; Zilong Zhao; Jie Liu; Liang Zhang; Hongjie Wang; Yongzhen Peng

doi:10.1016/j.biortech.2024.131792

Achieving efficient anammox contribution and the enrichment of functional bacteria in partial denitrification/anammox system: Performance, microbial evolution and correlation analysis

Zilong Hou
, Wenyi Dong
, Yanchen Li
, Shuo Chen
, Huaguang Liu
, Qi Han
, Zilong Zhao
, Jie Liu
, Liang Zhang
, Hongjie Wang^*
, Yongzhen Peng

^*Corresponding author for this work

Harbin Institute of Technology Shenzhen
School of Environment, Harbin Institute of Technology
Shenzhen Key Laboratory of Water Resource Utilization and Environmental Pollution Control
Chongqing University of Science and Technology
Shenzhen Wanmu Water Services Co
Beijing University of Technology

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

The primary challenge of applying partial denitrification/anammox (PD/A) to municipal wastewater treatment lied in the enrichment of functional bacteria with a considerable autotrophic nitrogen removal performance. The results showed influent NO₃⁻-N: NH₄⁺-N, reaction time and temperature would influence anammox nitrogen removal contribution. ¹⁵N isotopic tracing technology further revealed the average anammox contribution rate was up to 94.8 %. Extending reaction time was an effective measure to improve simultaneously PD and anammox activity. Microbial community indicated partial denitrifying bacteria (Bacillus) and anammox bacteria (Candidatus Brocadia) were enriched with abundance of 27.27 % and 7.09 % at NO₃⁻-N: NH₄⁺-N of 1:1. The correlation analysis showed that NO₃⁻-N: NH₄⁺-N ratio played the positive role for Bacillus enrichment, and low temperature was favorable to the enrichment of Thauera and Candidatus Jettenia. Overall, this study demonstrated the reasonable operational strategy would strengthen anammox contribution and facilitate enrichment of functional bacteria.

Original language	English
Article number	131792
Journal	Bioresource Technology
Volume	416
DOIs	https://doi.org/10.1016/j.biortech.2024.131792
State	Published - Jan 2025
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 7 Affordable and Clean Energy

Keywords

Anammox
Correlation analysis
Functional bacteria
Nitrogen removal contribution
Partial denitrification

Access to Document

10.1016/j.biortech.2024.131792

Cite this

Hou, Z., Dong, W., Li, Y., Chen, S., Liu, H., Han, Q., Zhao, Z., Liu, J., Zhang, L., Wang, H., & Peng, Y. (2025). Achieving efficient anammox contribution and the enrichment of functional bacteria in partial denitrification/anammox system: Performance, microbial evolution and correlation analysis. Bioresource Technology, 416, Article 131792. https://doi.org/10.1016/j.biortech.2024.131792

@article{6cef89e3dd59457892c36494a4d2ed59,

title = "Achieving efficient anammox contribution and the enrichment of functional bacteria in partial denitrification/anammox system: Performance, microbial evolution and correlation analysis",

abstract = "The primary challenge of applying partial denitrification/anammox (PD/A) to municipal wastewater treatment lied in the enrichment of functional bacteria with a considerable autotrophic nitrogen removal performance. The results showed influent NO3−-N: NH4+-N, reaction time and temperature would influence anammox nitrogen removal contribution. 15N isotopic tracing technology further revealed the average anammox contribution rate was up to 94.8 \%. Extending reaction time was an effective measure to improve simultaneously PD and anammox activity. Microbial community indicated partial denitrifying bacteria (Bacillus) and anammox bacteria (Candidatus Brocadia) were enriched with abundance of 27.27 \% and 7.09 \% at NO3−-N: NH4+-N of 1:1. The correlation analysis showed that NO3−-N: NH4+-N ratio played the positive role for Bacillus enrichment, and low temperature was favorable to the enrichment of Thauera and Candidatus Jettenia. Overall, this study demonstrated the reasonable operational strategy would strengthen anammox contribution and facilitate enrichment of functional bacteria.",

keywords = "Anammox, Correlation analysis, Functional bacteria, Nitrogen removal contribution, Partial denitrification",

author = "Zilong Hou and Wenyi Dong and Yanchen Li and Shuo Chen and Huaguang Liu and Qi Han and Zilong Zhao and Jie Liu and Liang Zhang and Hongjie Wang and Yongzhen Peng",

note = "Publisher Copyright: {\textcopyright} 2024",

year = "2025",

month = jan,

doi = "10.1016/j.biortech.2024.131792",

language = "英语",

volume = "416",

journal = "Bioresource Technology",

issn = "0960-8524",

publisher = "Elsevier Ltd",

}

Hou, Z, Dong, W, Li, Y, Chen, S, Liu, H, Han, Q, Zhao, Z, Liu, J, Zhang, L, Wang, H & Peng, Y 2025, 'Achieving efficient anammox contribution and the enrichment of functional bacteria in partial denitrification/anammox system: Performance, microbial evolution and correlation analysis', Bioresource Technology, vol. 416, 131792. https://doi.org/10.1016/j.biortech.2024.131792

TY - JOUR

T1 - Achieving efficient anammox contribution and the enrichment of functional bacteria in partial denitrification/anammox system

T2 - Performance, microbial evolution and correlation analysis

AU - Hou, Zilong

AU - Dong, Wenyi

AU - Li, Yanchen

AU - Chen, Shuo

AU - Liu, Huaguang

AU - Han, Qi

AU - Zhao, Zilong

AU - Liu, Jie

AU - Zhang, Liang

AU - Wang, Hongjie

AU - Peng, Yongzhen

PY - 2025/1

Y1 - 2025/1

N2 - The primary challenge of applying partial denitrification/anammox (PD/A) to municipal wastewater treatment lied in the enrichment of functional bacteria with a considerable autotrophic nitrogen removal performance. The results showed influent NO3−-N: NH4+-N, reaction time and temperature would influence anammox nitrogen removal contribution. 15N isotopic tracing technology further revealed the average anammox contribution rate was up to 94.8 %. Extending reaction time was an effective measure to improve simultaneously PD and anammox activity. Microbial community indicated partial denitrifying bacteria (Bacillus) and anammox bacteria (Candidatus Brocadia) were enriched with abundance of 27.27 % and 7.09 % at NO3−-N: NH4+-N of 1:1. The correlation analysis showed that NO3−-N: NH4+-N ratio played the positive role for Bacillus enrichment, and low temperature was favorable to the enrichment of Thauera and Candidatus Jettenia. Overall, this study demonstrated the reasonable operational strategy would strengthen anammox contribution and facilitate enrichment of functional bacteria.

AB - The primary challenge of applying partial denitrification/anammox (PD/A) to municipal wastewater treatment lied in the enrichment of functional bacteria with a considerable autotrophic nitrogen removal performance. The results showed influent NO3−-N: NH4+-N, reaction time and temperature would influence anammox nitrogen removal contribution. 15N isotopic tracing technology further revealed the average anammox contribution rate was up to 94.8 %. Extending reaction time was an effective measure to improve simultaneously PD and anammox activity. Microbial community indicated partial denitrifying bacteria (Bacillus) and anammox bacteria (Candidatus Brocadia) were enriched with abundance of 27.27 % and 7.09 % at NO3−-N: NH4+-N of 1:1. The correlation analysis showed that NO3−-N: NH4+-N ratio played the positive role for Bacillus enrichment, and low temperature was favorable to the enrichment of Thauera and Candidatus Jettenia. Overall, this study demonstrated the reasonable operational strategy would strengthen anammox contribution and facilitate enrichment of functional bacteria.

KW - Anammox

KW - Correlation analysis

KW - Functional bacteria

KW - Nitrogen removal contribution

KW - Partial denitrification

UR - https://www.scopus.com/pages/publications/85208533223

U2 - 10.1016/j.biortech.2024.131792

DO - 10.1016/j.biortech.2024.131792

M3 - 文章

C2 - 39522616

AN - SCOPUS:85208533223

SN - 0960-8524

VL - 416

JO - Bioresource Technology

JF - Bioresource Technology

M1 - 131792

ER -

Achieving efficient anammox contribution and the enrichment of functional bacteria in partial denitrification/anammox system: Performance, microbial evolution and correlation analysis

Abstract

UN SDGs

Keywords

Access to Document

Other files and links

Fingerprint

Cite this